南京农业大学学报2017，Vol.40Issue(2)：308-313,6.DOI:10.7685/jnau.201608028
注射促肾上腺皮质激素对母猪断奶后新生黄体的发育及类固醇合成的影响
Adrenocorticotropic hormone(ACTH) affects new corpus luteal development and steroidogenesis in weaned sows
摘要
Abstract
[Objectives] The purpose of the present study was to evaluate the impact of development and steroidogenesis of newly formed corpus luteal(CL) by stress in sows.[Methods] After injection of adrenocorticotropic hormone(ACTH) for simulating stress physiologic response,the tissue developmental status and the ability of steroidogenesis of the newly formed CL were examined by the RT-qPCR and immunohistochemistric methods.[Results] The results showed that plasma cortisol level in the ACTH treated sows was elevated significantly compared with the control.The expressions of P450 cholesterol side chain cleavage (P450scc) and 3β-hydroxysteroid dehydrogenase (3β-HSD)were down-regulated in the new CL of ACTH treated sows.The expressions of MMPs and VEGFa in the CL decreased compared to those of control sows.The ovary sections revealed a lower filling rate of luteal cells,and the capillary density in the CL was reduction in the ACTH treated sows.[Conclusions] The results suggested that the expression of main progesterone synthases and the angiogenesis of the newly formed CL in weaned sows could be impaired by higher plasma cortisol level because of ACTH administration.Stress can impact the normal development and function of the newly formed CL in sows.关键词
应激/外源促肾上腺皮质激素/断奶母猪/黄体发育/类固醇合成Key words
stress/ACTH administration/weaned sows/corpus luteal development/steroidogenesis分类
农业科技引用本文复制引用
钱庄,朱怀森,吕英军,刘红林,鲍恩东..注射促肾上腺皮质激素对母猪断奶后新生黄体的发育及类固醇合成的影响[J].南京农业大学学报,2017,40(2):308-313,6.基金项目
国家重点基础研究计划(973计划)资助项目(2014CB138502) （973计划）
