南京农业大学学报2017，Vol.40Issue(2)：339-345,7.DOI:10.7685/jnau.201602030
日粮添加亮氨酸对宫内发育迟缓断奶仔猪小肠葡萄糖吸收和能量代谢的影响
Effects of leucine supplementation on the small intestinal glucose absolution and energy metabolism in weanling piglets with intrauterine growth retardation
摘要
Abstract
[Objectives] The paper aims to investigate the effects of leucine on the small intestinal glucose absolution and energy metabolism in weanling piglets with intrauterine growth retardation(IUGR).[Methods] Sixteen normal birth weight(NBW) piglets and sixteen IUGR piglets were selected according to birth weight.At weaning(14 days of age),both NBW and IUGR piglets were fed control diet(containing 1.45％ leucine)or leucine diet(containing 1.80％ leucine).Then,all piglets were randomly distributed into 4 treatments:NC (NBW+control diet),NL (NBW+leucine diet),IC (IUGR +control diet) and IL (IUGR+leucine diet) for 21 d (n =8).Totally 8 piglets were selected from each group and slaughtered,and the jejunal enzyme activities and mRNA expression were measured.[Results] The results showed that the activities of AKP,Na+/K+-ATPase,PK and the content of ATP in the jejunum,and the activity of MDH in the jejunal mitochondria were markedly decreased in IUGR piglets (P＜ 0.05).A tendency toward decreased SGLT1 and PK mRNA expressions were observed in the jejunum,whilst IUGR significantly decreased AMPK-α1 and mTOR mRNA expressions(P＜0.05).Consumption of the leucine diet significantly increased the activity of AKP in the jejunum,and the activities of MDH and SDH in the jejunal mitochondria of IUGR piglets(P＜0.05),but Na+/K+-ATPase activity and ATP content in the jejunum showed a slight increase after leucine treatment,and there was a significant increase in SGLT1,GLUT2,AMPK-α1 and mTOR mRNA expressions(P＜0.05).[Conclusions] These results indicate that dietary leucine supplementation could improve the small intestinal development,and ameliorate small intestinal glucose absorption and energy metabolic status,by increasing the key enzyme activities and gene expressions of glucose absorption and energy metabolic effectively.关键词
亮氨酸/宫内发育迟缓/葡萄糖吸收/能量代谢/仔猪Key words
leucine/intrauterine growth retardation/glucose absolution/energy metabolism/piglets分类
农业科技引用本文复制引用
黄强,白凯文,徐稳,何进田,张莉莉,周乐,王恬..日粮添加亮氨酸对宫内发育迟缓断奶仔猪小肠葡萄糖吸收和能量代谢的影响[J].南京农业大学学报,2017,40(2):339-345,7.基金项目
国家重点基础研究发展计划项目(2012CB124703) （2012CB124703）
