农业工程学报2017，Vol.33Issue(4)：273-277,5.DOI:10.11975/j.issn.1002-6819.2017.04.037
养殖污水中蛋白核小球藻的分离鉴定及其污水处理效果
Isolation, identification ofChlorella pyrenoides from aquaculture wastewater and its purification of wastewater
摘要
Abstract
Algaehas been studied for many years about its characteristics of rapid breed, easily obtained, high adsorption capacity and low nutrition demands and so on.The emission of aquaculture wastewater will caused severe damage on environment.Therefore, how to remove the compound of nitrogen and phosphorus from aquaculture wastewater becomes a serious problem around the world.Based above, many researchers have been focusing on this field and a lot of achievements have been found. Many environmental factors could influence on the growth of algae, including temperature, illumination and nutrition. For heterotrophism, carbon, nitrogen and phosphorus are the three most important factors influencing on the growth of Chlorella pyrenoides. Microalgaeconsist of several varieties, includingCyanophyta, Chlorophyta, Chrysophyta and Rhodophyta.Chlorella pyrenoides is belonged toChlorophyta. Until now, studies ofChlorella pyrenoides have mainly focused on the processing, especially in the field of producing biodiesel, but few studies focused on the wastewater treatment byChlorella pyrenoides. This study carried out the following experiments: the enrichment, isolation, purification and nutritional ingredient analysis forChlorella pyrenoides. Then, the effect of treating with wastewater byChlorella pyrenoides was carried out, including the removal of total nitrogen, total phosphorus and reducing COD.Chlorella pyrenoideswas isolated from aquaculture wastewater with BG-11medium. The morphology and components ofChlorella pyrenoides were observed by phase contrast microscope and Fourier transform infrared spectrometer (FT-IR), respectively.The crystal structure ofChlorella pyrenoides was determined with X-ray. Protein and fat contents were measured by Kjeldahl determination and organic solvent extract method, respectively. The effect of treating with wastewater byChlorella pyrenoides was tested with national standard methods. Chlorophyll a was extracted and analyzed with acetone extract method. The morphology observation with phase contrast microscope showed thatChlorella was belonged toChlorella pyrenoides.FT-IR analysis showed that two peaks appeared at the wave numbers of 1080 and 1240 cm-1, which proved high contents of protein, carbohydrate and fat contained inChlorella pyrenoides. X-ray diffraction spectrogram showed two main peaks appeared at about 32° and 20°, which proved both protein and fat were contained inChlorella pyrenoides. Quantitative analysis indicated that contents of protein and fat were 45.6% and 8.1%, respectively. Correlational study indicated that the negative correlation of the increase of Chlorophyll a inChlorella pyrenoideswith the decrease of total nitrogen (T-N) and total phosphorus (T-P) remained in the wastewater. The removals of COD, total nitrogen (T-N) and total phosphorus (T-P) byChlorella pyrenoides were 70.9%, 23% and 34.7%, respectively. Based on above results, the capability of treating with wastewater byChlorella pyrenoideswasproved in this study. Therefore, this study could provide a reference for the exploitation of a new bioresource and the water environment treatment.关键词
污水/净化/氮/磷/蛋白核小球藻/筛选鉴定/成分分析/处理效果Key words
wastewaters/purification/nitrogen/phosphorus/Chlorella pyrenoides/screening and identification/component analysis/treatment effect分类
生物科学引用本文复制引用
陆洪省,刘亚樵,刘文君,孔凡民,谭好臣..养殖污水中蛋白核小球藻的分离鉴定及其污水处理效果[J].农业工程学报,2017,33(4):273-277,5.基金项目
山东省自然科学基金面上项目(No. ZR2014EMM005) （No. ZR2014EMM005）
山东省人力资源与社会保障厅资助项目(No. 20101008) （No. 20101008）
潍坊市峡山水库管理局资助项目(No. 201303201603). （No. 201303201603）
