农业生物技术学报2017，Vol.25Issue(2)：214-221,8.DOI:10.3969/j.issn.1674-7968.2017.02.005
黄肿树JcFATA基因组织表达特性及其启动子克隆与表达分析
Expression of JcFATA Gene in Jatropha curcas and Its Promoter Cloning and Analysis
摘要
Abstract
Fatty acyl acyl carrier thioesterase A (FATA) plays an important role in the process of fatty acid synthesis metabolism.The expression pattern of FATA of Jatropha curcas (JcFATA) and its promoter all has not been reported.To study the expression pattern of JcFATA gene,semi-quantitative RT-PCR and qRT-PCR were applied to detect the expression specificity of JcFATA gene.The results showed that JcFATA gene could express in multiple tissues of J.curcas,especially highly expressed in roots,flowers and leaves.In addition,in order to analyze the expression pattern of JcFATA gene promoter (PJcFATA),JcFATA gene promoter was cloned firstly and inserted into a binary expression vector pCAMBIA1300G,and then the fusion expression vector of [β-glucuronidase (GUS) gene drived by JcFATA gene promoter (pl300G-PJcFATA-GUS) was constructed successfully.The fusion expression vector was introduced into Arabidopsis thaliana wild type by Agrobacterium-mediated method.After effective selection for hygromycin B resistance,the resistant plants were detected by PCR and GUS histochemical analysis.PCR detection results showed that JcFATA gene promoter had been transferred into the genome of A.thaliana (JGD No.Jcr4S00539).Results of GUS histochemical staining of tissues in different development periods of A.thaliana revealed that GUS activity displayed obviously tissue specificity,with the deeper staining in roots,flowers and leaves.All above results paved the way to further function study ofJcFATA gene and JcFATA gene promoter.关键词
黄肿树脂酰-酰基载体蛋白硫酯酶A基因(JcFATA)/启动子/拟南芥转化/GUS组织化学染色Key words
J.curcas fatty acyl acyl carrier thioesterase A (JcFATA) gene/Promoter/Arabidopsis thaliana transformation/GUS histochemical staining分类
农业科技引用本文复制引用
刘颖,杨亚利,殷学贵,李林峰,朱宏波,陆建农,施玉珍..黄肿树JcFATA基因组织表达特性及其启动子克隆与表达分析[J].农业生物技术学报,2017,25(2):214-221,8.基金项目
国家自然科学基金项目(No.31271759)和湛江市科技攻关项目(No.2016B101) （No.31271759）
