农业生物技术学报2017，Vol.25Issue(2)：335-344,10.DOI:10.3969/j.issn.1674-7968.2017.02.019
环介导等温扩增法检测6种转基因大豆
Detection of Six Kinds of Genetically Modified Soybean (Glycine max) by LAMP Method
摘要
Abstract
With the increasing of the global commercial production of genetically modified products,the detection of transgenic lines has gradually become the development trend of the detection of genetically modified components in internal and international.To carry out the regulations of genetically modified organisms in China's inward and outward,environmental release and labeling,and achieve effective management of genetically modified soybean (Glycine max),the loop-mediated isothermal amplification (LAMP) primers were designed according to the flanking sequence of genetically modified soybean DAS-81419-2,DP356043-5,A2704-12,FG72,BPS-CV 127-9 and MON89788,separately.Through specificity,sensitivity and limit of detection testing,an LAMP method for the inspection of genetically modified soybean DAS-81419-2,DP356043-5,A2704-12,FG72,BPS-CV127-9 and MON89788 was developed.The results showed that this method could detect genetically modified soybean DAS-81419-2,DP356043-5,A2704-12,FG72,BPS-CV127-9 and MON89788 specifically and stably with a low detection limit of 0.1％ (W/W).Since the LAMP detection could be finished within 30 min and the results could be directly detected according to the color change produced by SYBR Green Ⅰ dye with the naked eye.This method allowed rapid and accurate detection of genetically modified products on the inspection agency and relevant law enforcement departments,and non-essential of complex and expensive equipment.关键词
转基因大豆品系/环介导等温扩增(LAMP)/特异性/灵敏度/检出限/准确性Key words
Genetically modified soybean/Loop-mediated isothermal amplification (LAMP)/Specificity detection/Sensitivity detection/Limit of detection/Accuracy分类
农业科技引用本文复制引用
周杰,黄文胜,邓婷婷,吴亚君,胡秋辉,陈颖..环介导等温扩增法检测6种转基因大豆[J].农业生物技术学报,2017,25(2):335-344,10.基金项目
国家转基因生物新品种培育重大专项“转基因生物抽制样和精准检测技术项目”(No.2014ZX08012-001) （No.2014ZX08012-001）
