热带作物学报2017，Vol.38Issue(1)：70-75,6.DOI:10.3969/j.issn.1000-2561.2017.01.013
巴西橡胶树PEPRK基因家族成员的鉴定和表达分析
Identification and Expression of PEPRK Genes from Hevea brasliensis
摘要
Abstract
Phosphoenolpyruvate carboxylase kinase-related kinase is a member of CDPK-SnRK superfamily.Little is known about its functions.By searching the transcriptome and genome databases,we have identified 25 PEPRK genes from Hevea brasliensis and five other plant species,including five members from H.brasliensis,which were named HbPEPRK1 to 5.Sequence analysis revealed that all PEPRK genes comprised one protein kinase domain.The plant PEPRK clustered into two major groups in phylogenetic tree,the members in the same group shared a similar introns and domains distribution.H.brasliensis PEPRKs were more closely related to PEPRKs of Manihot esculenta.The results of expression analysis indicated that HbPEPRK1 was mainly expressed in mature leaves,HbPEPRK2 and HbPEPRK5 were expressed in leaves,bark and latex,HbPEPRK4 was mainly expressed in seeds.In addition,the expression of H.brasliensis PEPRK genes was related to leaf development,ethephon stimulation,C.cassiicola infection and low temperature.关键词
巴西橡胶树/PEPRK/基因家族/结构与进化/表达分析Key words
Hevea brasiliensis/PEPRK/gene family/structure and evolution/expression analysis分类
农业科技引用本文复制引用
肖小虎,隋金蕾,戚继艳,方永军,周斌辉,唐朝荣..巴西橡胶树PEPRK基因家族成员的鉴定和表达分析[J].热带作物学报,2017,38(1):70-75,6.基金项目
国家自然科学基金项目(No.31400565). （No.31400565）
