热带作物学报2017，Vol.38Issue(1)：83-88,6.DOI:10.3969/j.issn.1000-2561.2017.01.015
干旱胁迫下香蕉miRNAs的表达分析
The Expression Analysis of Banana miRNAs Under Drought Stress
摘要
Abstract
The miRNAs expression of drought-acclimated and non-acclimated banana plantlets were researched under the drought stress by Real-time PCR.The experimental results showed that the expression of six miRNAs were detected in all plantlets under the drought stress,but the expression patterns of these miRNAs were differential.The expression profiles of miR156k,miR160a,miR162a,miR164a and miR166d were increased first and decreased later,and the expression pattem of miR397b showed a tendency of increase first then decrease,again rise again fall in the non-acclimated banana plantlets.On the other hand,in the drought-acclimated banana plantlets,the expression profiles of miR156k,miR162a,miR166d and miR397b were increased first and decreased then,and the expression patterns of miR160a and miR164a showed a tendency of increase first then decrease,again rise again fall.At all time points except for the 10th day after drought treatment,the expressional levels of most miRNAs in the acclimated banana plantlets were higher than those in the non-acclimated plantlets,and the expressional levels of miR160a and miR164a were higher than those of the other four miRNAs in any plantlets at any time points.These research findings should lay a better foundation for the further research of drought-stress response mechanism in banana.关键词
香蕉/干旱胁迫/miRNAs/表达分析Key words
bananas/drought stress/miRNAs/expression analysis分类
农业科技引用本文复制引用
张恒,冯仁军,王静毅,云天艳,李成梁,张银东,张锡炎..干旱胁迫下香蕉miRNAs的表达分析[J].热带作物学报,2017,38(1):83-88,6.基金项目
国家自然科学基金项目(No.31201603) （No.31201603）
海南省自然科学基金项目(No.312051) （No.312051）
国家863计划项目子课题(No.2012AA101204-2) （No.2012AA101204-2）
海南省科技合作专项资金项目(No.KJHZ2015-26) （No.KJHZ2015-26）
现代农业产业技术体系建设专项(No.CARS-32). （No.CARS-32）
