热带作物学报2017，Vol.38Issue(1)：104-110,7.DOI:10.3969/j.issn.1000-2561.2017.01.018
王氏唇柱苣苔的离体培养及遗传稳定性
In vitro Rapid Propagation of Chirita wangiana and Genetic Stability
摘要
Abstract
In vitro rapid propagation of Chirita wangiana using the leaf section as the explant was studied,and the genetic stability of the regenerated plantlets was analyzed by flow cytometry and chromosome number.The results showed that the most optimal bud induction medium was MS medium supplemented with 0.5 mg/L 6-BA and 0.1 mg/L NAA;the suitable subculture medium was MS medium with 0.5 mg/L 6-BA and 0.05 mg/L NAA;1/2 MS medium supplemented with 15 g/L sucrose and 0.5 mg/L IBA was suitable for rooting.Histological investigation revealed that the buds and plantlets regenerated through organogenesis.Ploidy investigation by flow cytometry showed that there was no ploidy variation among the regenerated plantlets,though there was only a 1.86％ decrease in the genome size.Both the mother plant and in vitro regenerated plantlets had the same chromosome numbers (2n=36) and unchanged morphological characteristics.The results could be used for large scale rapid propagation of genetic stable seedlings of C.wangiana in horticultural application.关键词
王氏唇柱苣苔/组织培养/流式细胞术/染色体数Key words
Chirita wangiana/tissue culture/flow cytometry/chromosome number分类
农业科技引用本文复制引用
李谦盛,赵伟,沈娟,周纯亮..王氏唇柱苣苔的离体培养及遗传稳定性[J].热带作物学报,2017,38(1):104-110,7.基金项目
上海市自然科学基金(No.14ZR1441000) （No.14ZR1441000）
