热带作物学报2017，Vol.38Issue(1)：136-143,8.DOI:10.3969/j.issn.1000-2561.2017.01.024
不同地区荷花腐败病菌生物学特性及IGS序列分析
Biological Characteristics and IGS Sequence Analysis of Pathogen Strains Causing Lotus Rhizome Rot(Fusarium commune)from Different Regions
摘要
Abstract
Rhizome rot disease of lotus,which had occurred widely,not only caused severe damage to lotus production,but also became one of the major obstacles to the sustaining development of lotus industry in China.To clarity the biological characteristics and genetic differentiation of Fusarium commune,the causal agent of lotus rhizome rot,pathogenicity test,biological factors (temperature,pH value,carbon and nitrogen sources) and phylogenetic analysis based on rDNA IGS gene were studied.The results showed that F.commune could grow from 15 to 35 ℃,and the optimal temperature was 25 ℃.It grew well from pH 4 to 9,and the optimal pH was 7.It could also utilize many substances as carbon and nitrogen sources.Among tested sucrose,saccharose and glucose were the best carbon sources for the growth of F.commune,peptone was the most suitable nitrogen source while ureal was the worst.When the cultivation temperature was 15,20,30 or 35 ℃,Hubei isolates had the largest colony diameter.Guangdong isolates had the largest colony diameter from pH 4 to 5,and Hubei isolates had the largest colony diameter from pH 7 to 9.Pathogenicity differentiation was existed between pathogen strains from different regions.Phylogenetic analysis showed that there was obvious genetic differentiation between these isolates from different provinces.Guangdong isolates has the similarity of 99.7％ to Hunan isolates and the genetic distance between two provinces was 0.003.Whereas,Guangdong isolates presented 97.3％ similarity to Hubei isolates and the genetic distance between two provinces was 0.027.关键词
荷花腐败病/病原菌/生物学特性/rDNA-IGS序列Key words
lotus rhizome rot disease/pathogen/biological characteristics/rDNA IGS sequence分类
农业科技引用本文复制引用
曾莉莎,吕顺,郑芝波,梁少丽,周建坤,庄华才,杜彩娴,麦进培,莫秀文..不同地区荷花腐败病菌生物学特性及IGS序列分析[J].热带作物学报,2017,38(1):136-143,8.基金项目
广东省科技计划项目(No.2015A020209002) （No.2015A020209002）
东莞市社会科技发展项目(No.2014108101044). （No.2014108101044）
