热带作物学报2017，Vol.38Issue(2)：295-301,7.DOI:10.3969/j.issn.1000-2561.2017.02.018
巴西橡胶树HbSERK1启动子的克隆及功能鉴定
Molecular and Functional Characterization of the HbSERK1 Promoter in Hevea brasiliensis
摘要
Abstract
The 5'regulatory region of HbSERK1 from Hevea brasiliensis was cloned using GenomeWalker strategy.A/T content in this sequence is up to 66.2％,and meet the characteristic of eukaryotic promoter sequence.TATA box,CAAT box CAT-box,02-site,ERE,ARE,TCA-element,GCN4-motif,ACA-motif were found in this promoter region.In order to verify HbSERK1 promoter function,Five deletions plant expression vector were constructed and transformed to the tobacco by Agrobacterium-mediated vacuum infiltration method and Agrobacterium-mediated.GUS activity analysis revealed that the region from-1 395～-252 bp nt drived expression of the GUS gene except SP5 deletion (-252 bp).The results of transient expression and stable expression suggest HbSERK1 promoter possess bioactivity.关键词
巴西橡胶树/HbSERK1启动子/功能鉴定Key words
Hevea brasiliensis/HbSERK1 promoter/functional characterization分类
农业科技引用本文复制引用
王颖,贾瑞,李辉亮,郭冬,朱家红,陈雄庭,彭世清..巴西橡胶树HbSERK1启动子的克隆及功能鉴定[J].热带作物学报,2017,38(2):295-301,7.基金项目
海南省自然科学基金(No.314115) （No.314115）
星火计划重点项目(No.2014GA800003). （No.2014GA800003）
