热带作物学报2017，Vol.38Issue(2)：335-342,8.DOI:10.3969/j.issn.1000-2561.2017.02.023
香蕉枯萎病菌4号生理小种cat1基因敲除与表型分析
Gene Knockout and Phenotype Analysis of the catalase-1 in Fusarium oxysporum f.sp.cubense Race 4
摘要
Abstract
It was found that the cat1 gene in Fusarium oxysporum f.sp.cubense race 4 (Foc4) was up-regulated induced by Musa sp.AAA.cv.Baxi in our previous proteomics analysis.In order to investigate the function of cat1 during the host infection process of Foc4,the cat1 gene in Foc4 was deleted by the homologous recombination method.The phenotype and pathogenicity of the cat1 knockout mutants were then analyzed.The results indicated that there were no apparent difference between the mutants and the wild type strain on mycelium and spores morphology,colonial growth,osmotic stress resistance and cell wall selective pressure.However,the mutants showed significant decrease in the abilities of exogenous oxygen stress resistance,penetrability of cell wall,cellulose utilization,and the pathogenicity to Musa spp.AAA.cv.Baxi.These results suggested that the cat1 gene in Foc4 should be involved in pathogenesis by scavenging host-derived ROS,and participated in the process of host cellulose degradation.关键词
巴西蕉/尖孢镰刀菌古巴专化型/cat1基因/基因敲除/致病性Key words
Musa spp.AAA.cv.Baxi/Fusarium oxysporum f.sp.cubense/cat1 gene/knockout/pathogenicity分类
农业科技引用本文复制引用
王小琳,李春强,杨景豪,李文彬,孙建波,彭明..香蕉枯萎病菌4号生理小种cat1基因敲除与表型分析[J].热带作物学报,2017,38(2):335-342,8.基金项目
海南耕地改良关键技术研究与示范专项(No.HNGDXF2015) （No.HNGDXF2015）
中国热带农业科学院热带生物技术研究所基本科研业务费(No.ITBB2015ZT02) （No.ITBB2015ZT02）
国家自然科学基金(No.31271997) （No.31271997）
公益性行业农业科研专项(No.201503110). （No.201503110）
