热带作物学报2017，Vol.38Issue(2)：343-348,6.DOI:10.3969/j.issn.1000-2561.2017.02.024
保护酶CAT在木薯种质抗螨中的功能初步研究
Preliminary Study of The Function of Catalase in Cassava Resistance to Mite
摘要
Abstract
In order to validate the function of catalase (CAT) in cassava resistance to mite,we determined the changes of transcriptions and activities of CAT in either cassava or Tetranychus cinnabarinus after the miteresistant and mite-susceptible cassava cultivars were damaged by T.cinnabarinus.The results showed that,in one hand,compared to the same leaves before damaged by T.cinnabarinus,the transcriptions of MeCAT1 and total activities of CAT in leaves of 1 d and 8 d-damaged mite-susceptible cassava cultivar BRA900 was 1.11-and 1.24-fold,1.06-and 1.15-fold,respectively,while the corresponding multiples of transcriptions in 1 d and 8 d-damaged leaves of mite-resistant cassava cultivar C1115 was 2.75-and 2.62-fold,and was 2.36-and 2.38-fold of total activities,respectively,which were both significantly higher than the levels of BRA900.In the other hand,when T.cinnabarinus feeding on BRA900 for 1 d and 8 d,the transcriptions of TcCAT and activities of CAT in T.cinnabarinus was 1.13 and 0.98-fold,1.02-and 0.99-fold compared to those before feeding,respectively,while the corresponding multiples of transcriptions in T.cinnabarinus feeding on C1115 was 0.57-and 0.61-fold,and were 0.52-and 0.61-fold of total activities,respectively,which were both significantly lower than the levels of BRA900.These results preliminarily demonstrated that the induction of CAT could release oxidative damage caused by T.cinnabarinus,and the suppression of CAT could inhibit the feeding adaption of T.cinnabarinus,which both contribute to cassava resistance to mite.This study provides a theoretical basis for molecular breeding of mite-resistance cassava.关键词
CAT/朱砂叶螨/木薯种质/抗螨功能Key words
Catalase/Tetranychus cinnabarinus/cassava/resistance to mite分类
农业科技引用本文复制引用
梁晓,卢芙萍,卢辉,伍春玲,何宏力,陈青,朱俊洪..保护酶CAT在木薯种质抗螨中的功能初步研究[J].热带作物学报,2017,38(2):343-348,6.基金项目
国家木薯产业技术体系害虫的防控岗位科学专项(No.CARS-12-hncq). （No.CARS-12-hncq）
