水产学报2017，Vol.41Issue(3)：321-329,9.DOI:10.11964/jfc.20160410359
5种鳎科鱼类核糖体ITS1序列比较
Comparative analysis of the first internal transcribed spacer of ribosomal DNA in five Soleidae species
摘要
Abstract
For a long time,the ribosomal RNA gene was thought to conform to the paradigm of strict concerted evolution pattern.In fact,an increasing number of intraindividual and intraspecies variations have been discovered,indicating non-concerted evolution.In order to explore the polymorphism of the first internal transcribed spacer (ITS1) in the species of Soleidae in Pleuronectiformes and whether it is suitable as a marker for species identification,a total of 118 ITS 1 sequences were determined from five soles.The results showed that two distinct types were found in Pardachirus pavoninus genome,suggesting a non-concerted evolution pattern,while concerted evolution was confirmed in other four soles because of absence of intraindividual polymorphism.Sequence analysis demonstrated that ITS 1 had significant length heterogeneity,ranging from 412 bp in Zebrias quagga to 585 bp in P.pavoninus.The base content of sequences in all five soles was in the same trend with C＞G＞A＞T and GC content (69.5％) was far higher than AT content.Except for one sequence of P.pavoninus,each of five soles clustered into one clade,respectively,suggesting ITS1 sequence was suitable for species identification in the present study.It is worth noting that the sequence of P.pavoninus clustered with Brachiru orientalis,suggesting that ITS 1 sequence polymorphism interfered species identification,as a result,it is essential to obtain enough clones before using ITS 1 sequence as the molecular marker for species identification in order to avoid incorrect conclusions.关键词
鳎科/内转录间隔区/种类鉴定/分子标记/假基因/非协同进化/聚类分析Key words
Soleidae/internal transcribed spacer/species identification/molecular marker/pseudogene/non-concerted evolution/cluster analysis分类
生物科学引用本文复制引用
龚理,时伟,杨敏,司李真,孔晓瑜..5种鳎科鱼类核糖体ITS1序列比较[J].水产学报,2017,41(3):321-329,9.基金项目
National Natural Science Foundation of China (31272273) （31272273）
Open Research Fund Program of Guangxi Key Lab of Mangrove Conservation and Utilization (GKLMC-201502)国家自然科学基金(31272273) （GKLMC-201502）
广西红树林保护与利用重点实验室开放基金(GKLMC-201502) （GKLMC-201502）
