水产学报2017，Vol.41Issue(3)：330-338,9.DOI:10.11964/jfc.20160410379
团头鲂耐低氧新品系雌核发育群体遗传结构的微卫星分析
Analysis of genetic structure of gynogenetic population in new strain of hypoxia-tolerant Megalobrama amblycephala using microsatellite markers
摘要
Abstract
To guide the subsequent breeding of hypoxia-tolerant population of Megalobrama amblycephala;genetic structures of hypoxia-tolerant (TN),meiotic gynogenesis (TNM),mitotic gynogenesis populations (TNDH)of M.amblycephala were analyzed using twenty microsatellite markers in this study.The mean value of number of alleles (Na) of M.amblycephala TN,TNM,TNDH and the control TPJ1 was 3.90,3.55,3.45 and 2.56,respectively.The mean value of observed heterozygosity (Ho) was 0.7853,0.3934,0.2768 and 0.8075,respectively.The mean value of expected heterozygosity (He) was 0.6491,0.5563,0.4870 and 0.6855,respectively.And the average value of polymorphism information content (PIC) was 0.5695,0.4796,0.4181 and 0.6105,respectively.It demonstrated that the genetic diversity of TPJ1 was the highest,and TN population had lower genetic diversity,but there is no significant difference and there was the high genetic heterozygosity.The genetic diversity of TNDH population was significantly lower than TNM population,and the genetic diversity of the gynogenetic (TNM and TNDH) was significantly lower than TPJ1 and TN populations.The mean HardyWeinberg index of the TPJ1 and TN had heterozygote excess and the gynogenetic populations (TNM and TNDH)showed heterozygote deficit.Using unweighted pair-group method with arithmetic means method (UPGMA)based on their genetic distances,TPJ1 population and TN population were grouped in one cluster,while TNM population and TNDH population were classified into another cluster,indicating a genetic differentiation between the two clusters.Our results show that the hypoxia-tolerant group in M.amblycephala (TN) in gynogenesis can accelerate homozygosity of genetic material and will have further purification of M.amblycephala hypoxiatolerant genes.关键词
团头鲂/耐低氧群体/雌核发育/遗传结构/微卫星标记Key words
Megalobrama amblycephala/hypoxia-tolerant/gynogenesis/genetic structure/microsatellite分类
生物科学引用本文复制引用
徐湛宁,李福贵,郑国栋,陈杰,蒋霞云,邹曙明..团头鲂耐低氧新品系雌核发育群体遗传结构的微卫星分析[J].水产学报,2017,41(3):330-338,9.基金项目
The Key Technologies Research and Development Program of China (2012BAD26B00) （2012BAD26B00）
National Science Foundation of China (31272633,31201760) （31272633,31201760）
Shanghai University Knowledge Service Platform (ZF1206)国家科技支撑计划(2012BAD26B00) （ZF1206）
国家自然科学基金(31272633,31201760) （31272633,31201760）
上海高校知识服务平台(ZF1206) （ZF1206）
