水产学报2017，Vol.41Issue(3)：339-346,8.DOI:10.11964/jfc.20140709398
凡纳滨对虾引进群体与养殖群体遗传多样性的AFLP分析
AFLP analysis of genetic diversity of imported Litopenaeus vannamei populations and cultured stocks
摘要
Abstract
The white shrimp,Litopenaeus vannamei,is one of the most important aquaculture species in China.The broodstocks used in China are mainly introduced from abroad.Recently,several new domestic white shrimp strains have been bred and used as broodstock.To further strengthen the genetic breeding of white shrimp,it is necessary to understand genetic diversity status of different strains.In the present study,the amplified fragment length polymorphism (AFLP) was employed to analyze the population genetic diversity and genetic difference between four introduced populations and three domestic breeding populations.A total of 105 fragments in length of 100～500 bp were identified from 210 individuals using seven AFLP primer combinations,of which 90 of 105 fragments were polymorphic,and the proportion of polymorphic loci was 85.71％.The polymorphic loci within populations varied from 54.17％ to 65.63％,with an average of 60.53％,and the mean heterozygosities ranged from 0.164 to 0.236,with an average of 0.202.AMOVA analysis revealed that 51.79％ of genetic variations derived from populations,the estimated ΦST value over all polymorphic loci across the seven populations was 0.518,indicating significant genetic differences between populations and strong population structure.Furthermore,these results could provide theoretical supports for germplasm improvement ofL.Vannamei..关键词
凡纳滨对虾/遗传多样性/遗传差异/扩增片段长度多态性Key words
Litopenaeus vannamei/genetic diversity/genetic differences/AFLP分类
生物科学引用本文复制引用
叶宁,包秀凤,刘建勇..凡纳滨对虾引进群体与养殖群体遗传多样性的AFLP分析[J].水产学报,2017,41(3):339-346,8.基金项目
Guangdong Province Project of China (2010B020201014) （2010B020201014）
Special Funds for Promotion of Marine Fisheries of Guangdong Province (A200899E04,A201001H05,A201101B05)广东省科技计划(2010B020201014) （A200899E04,A201001H05,A201101B05）
广东省海洋与渔业局海洋渔业推广专项(A200899E04,A201001H05,A201101B05) （A200899E04,A201001H05,A201101B05）
