水产学报2017，Vol.41Issue(3)：347-357,11.DOI:10.11964/jfc.20160510396
香港牡蛎新型LRR受体克隆与功能分析
Cloning and function analysis of novel LRRs receptor in Crassostrea hongkongensis
摘要
Abstract
Leucine-rich repeats protein is a versatile ligand recognition domain present in receptors,which is involved in many important physiological processes such as development,immune responses and hormone regulation etc.A novel LRRs protein with significant high expression level during embryogenesis was identified in this study.Its function was investigated by various methods including cloning,sequences alignment,in situ hybridization and in vitro treatment with recombined proteins.SMART analysis indicated that an IG-like domain was located in the extracellular region besides the LRRs domain.Thus we designated it as Crassostrea hongkongensis LRRs and IG-like containing receptor (ChLIGR).Sequences alignment revealed that ChLIGR is a new member in the IG-like containing LRR proteins superfamily,and most of its homologous proteins belong to mollusk without any function related description.Consistent with its expression pattern during embryogenesis,the in situ hybridization results showed that ChLIGR was expressed most highly in blastula stage and was distributed to a distinct place in the embryo.Combining with the predicted ETS/AP-1 regulatory site upstream the promoter,we hypothesized that ChLIGR would be involved in gastrulation related epithelial-mesenchymal transition process.Notably,in vitro treatment with recombined extracellular domains of ChLIGR augments the expression of migration related genes,which partly supports our hypothesis.In conclusion,the function of this novel IG-like domain containing LRR receptor is preliminarily unveiled as an activator for gastrulation by promoting the cell migration.关键词
香港牡蛎/亮氨酸富集重复/胚胎发育/细胞迁移Key words
Crassostrea hongkongensis/leucine-rich repeats/embryogenesis/cell migration分类
生物科学引用本文复制引用
刘映,李军,张跃环,张扬,喻子牛..香港牡蛎新型LRR受体克隆与功能分析[J].水产学报,2017,41(3):347-357,11.基金项目
National Science Foundation of China (31502193) （31502193）
Guangdong Natural Science Foundation (2014A030310402) （2014A030310402）
Science and Technology Planning Project of Guangdong Province,China (2014B030301064)国家自然科学基金(31502193) （2014B030301064）
广东省自然科学基金博士启动项目(2014A030310402) （2014A030310402）
广东省科技计划项目(2014B030301064) （2014B030301064）
