水产学报2017，Vol.41Issue(3)：374-381,8.DOI:10.11964/jfc.20160210281
盐度胁迫对三疣梭子蟹肌肉和血淋巴中游离氨基酸含量的影响
Effects of different salinity level on free amino acid composition in muscle and hemolymph of the swimming crab Portunus trituberculatus
摘要
Abstract
Salinity is one of the most important factors influencing distribution,abundance and the physiology of aquatic animals.Changes in ambient salinity are directly related to osmoregulation capacity.During acclimation to salinity,the main challenge for aquatic animals is to regulate their osmotic pressure to maintain normal life activities.As the predominant aquatic animal,crustaceans can dwell in seawater and flesh water due to their various osmoregulatory mechanisms.The two forms of osmoregulatory mechanisms are anisosmotic extracellular regulation (AER) and intracellular isosmotic regulation (ⅡR).AER can mainly control the osmolality of the body fluid through regulation of gills and antennal glands,while ⅡR maintains intracellular osmolality and maintain the balance between tissues and the hemolymph primarily by regulating amino acids (FAAs) especially non-essential amino acids (NEAA).Under excessive changes in environmental salinity,it's difficult for body fluid osmolality to be maintained at a completely stable level through AER.At that point,it's believed that ⅡR compensates for AER by accumulating or degrading some particular NEAA as osmolytes,effectivley moderating fluctuations in extracellular osmolality and facilitating volume readjustment.Hemolymph is directly involved in responding to salinity exposure and muscle is the major tissue for protein deposition and possibly represents the main pool of amino acids.Therefore muscle tissue and hemolymph are very important to study FAAs's involvement in osmotic pressure regulation.The experiment analyses the free amino acids content and concentration variation in muscle and hemolymph of Portunus trituberculatus at different salinities.Clarify the composition of FAAs and its function in the salinity adaptation.Enrich the FAAs in the field of salinity adaptation and provide the basis for further study of the molecular mechanism.The contents of FAAs were measured at salinities and the experiment lasted for 5 days.Determination of FAAs extraction solution of muscle and hemolymph with Hitachi 835-50 automatic amino acid analyzer.Results showed that the highest amounts of individual FAA in seawater-adapted P.trituberculatus was exhibited by Tauine,Arg,Gly,Pro and Ala.As salinity increased,the total flee amino acids (TOFAA) of the hemolymph and muscle increased significantly.NEAA increased significantly when the salinity varied from 10 to 50 while Essential FAA (EAA) was not affected by external salinity change.The result showed that FAAs played an important role in salinity adaptation,especially Pro,Ala,Gly,Asp and Glu.Because Ala,Gly,Asp,Glu belong to the taste amino acids (TAA),so TAA played an important role in osmoregulation.The content of Pro increased significantly both in muscle and hemolymph,especially from 40 to 50 period.Therefore Pro played the most important role in hyperosmotic stress.关键词
三疣梭子蟹/血淋巴/肌肉/盐度/非必需氨基酸(NEAA)/鲜味氨基酸(TAA)/脯氨酸(Pro)Key words
Portunus trituberculatus/hemolymph/muscle/salinity/NEAA/TAA/Pro分类
农业科技引用本文复制引用
付萍,吕建建,刘萍,李健,高保全..盐度胁迫对三疣梭子蟹肌肉和血淋巴中游离氨基酸含量的影响[J].水产学报,2017,41(3):374-381,8.基金项目
National Natural Science Foundation of China (41576147) （41576147）
National Natural Science Foundation of China (41306177) （41306177）
Taishan Leading Talent Project Eco-efficient Agriculture Innovation Project (LJNY2015002)国家自然科学基金面上项目(41576147) （LJNY2015002）
国家自然科学基金青年基金(41306177) （41306177）
泰山领军人才工程高效生态农业创新类计划(LJNY2015002) （LJNY2015002）
