四川医学2017，Vol.38Issue(2)：130-135,6.DOI:10.16252/j.cnki.issn1004-0501-2017.02.004
不同剂量乳化异氟醚延迟预处理对大鼠心肌缺血再灌注损伤的保护作用
Protective Effect of Different Dose of Emulsified Isofluranedelayed Preconditioning on Myocardial Ischemia Reperfusion Injury in Rats
摘要
Abstract
Objective To evaluate the protective effects of different dose of emulsified isoflurane delayed preconditioning on myocardial ischemia reperfusion injury in rats and to investigate possible mechanism. Methods SD rats were randomized into sham group,IR group,IL group and EIso group. Samevolume of 0. 9% saline,30% fat emulsion and emulsified isoflurane 2,4,8 mL/kg( volume ratio of liquid isoflurane was 8%) were injected into the caudal vein in each group in 30 minutes. 24 hoursafter injection, the left anterior descending coronary artery was ligated for 45 minutes,then reperfusion for 3 hours,and the myocardial ischemiareper-fusion injury model was established. At the end of the experiment, the myocardial infarction size and lipid peroxidation index were measured by TTC method. The levels of plasma endothelin( ET) and tumor necrosis factor( TNF-α) were measured by radioimmunoas-say. TNF-αin myocardia cells was determined by immunohistochemistry. And the protective effects of emulsified isoflurane delayed preconditioning and possible inflammatory mechanisms were investigated. Results Compared with the model group,the infarction size was significantly decreased in emulsified isoflurane(4,8 mL /kg)group(P<0. 05),and the decrease was dose-dependent. Compared withemulsified isoflurane(2mL/kg)group,the infarction size in the emulsified isoflurane(8mL/kg)groupdecreased more significantly (P<0. 05). Compared with the sham group,the plasm level of lactate dehydrogenase(LDH)in the model,fat emulsion and the emul-sified isoflurane group increased significantly(P<0. 01). Compared with the model group,the level of LDH was significantly lower in emulsified isoflurane group(4,8mL/kg). And the endothelin level in emulsified isoflurane group(4,8mL/kg)was different from that in the model and fat emulsion group,with statistical significance(P<0. 05). The plasma concentration of TNF-αin emulsified isoflu-rane group(4,8mL/kg)significantly decreased(P<0. 05). Conclusion Delayed preconditioning with emulsified isoflurane had pro-tective effect on myocardial ischemiareperfusion injury in rats. The protective effect of emulsified isoflurane was dose-dependent. And the mechanism might be related to the inhibition of myocardial lipid peroxidation and inflammatory reaction.关键词
乳化异氟醚/大鼠/心肌/缺血再灌注损伤Key words
emulsified isoflurane/rat/myocardium/ischemia reperfusion injury分类
医药卫生引用本文复制引用
杨帅,胡朝阳,刘进,罗朝志..不同剂量乳化异氟醚延迟预处理对大鼠心肌缺血再灌注损伤的保护作用[J].四川医学,2017,38(2):130-135,6.基金项目
国家自然科学基金资助(编号:30901412) （编号:30901412）
