山东医药2017，Vol.57Issue(11)：15-18,4.DOI:10.3969/j.issn.1002-266X.2017.11.005
尾静脉注射不同剂量羟基红花黄色素A的缺血再灌注大鼠脑组织3-NT、iNOS、NO表达观察
Expression of 3-nitrotyrosine, iNOS and NO in brain tissues of ischemia-reperfusion rats treated with different doses of hydroxysafflor yellow A
摘要
Abstract
Objective To observe the expression changes of 3-nitrotyrosine (3-NT), inducible nitric oxide synthase (iNOS), and nitric oxide (NO) in brain tissues of ischemia-reperfusion rats treated with different doses of hydroxysafflor yellow A (HSYA) by intravenous injection.Methods The healthy adult male SD rats were randomly divided into the sham operation group, model group, low-dose HSYA group, medium-dose HSYA group and high-dose HSYA group.In the sham operation group, rats' common carotid artery, internal carotid artery and external carotid artery were exposed, and then sutured.The other rats were subjected to a 60-min middle cerebral artery occlusion and 24-h reperfusion (MCAO/R).The rats in the low-dose, medium-dose and high-dose HSYA groups were injected with 2.5, 5 and 10 mg/kg HSYA, respectively, in the tail vein at 60 min after ischemia, and the same volume of Tris buffer was injected into the tail vein of rats in sham operation group and model group.All rats were continued to raise for 24 h.The expression of 3-NT and iNOS in the rat brain tissues after ischemia-reperfusion in each group was examined by Western blotting.The NO level was also detected by Griess assay.Results The absolute gray values of 3-NT in the sham operation group, the model group, the low-dose HSYA group, the medium-dose HSYA group and the high-dose HSYA group after ischemia-reperfusion were (2.81±1.37)×103, (46.86±4.75)×103, (44.51±4.13)×103, (13.88±2.98)×103, and (6.38±2.66)×103, respectively;the absolute gray values of iNOS of each group were (2.25±0.32)×103, (79.67±4.73)×103, (75.29±4.08)×103, (27.31±2.77)×103, (19.19±1.86)×103, respectively.In the model group and low-dose, medium-dose, and high-dose HSYA groups, the levels of NO were (16.50±2.20), (15.40±1.44), (10.33±1.30), and (6.80±0.73) nmol/mg.The levels of 3-NT, iNOS and NO in brain tissues of the model group, low-dose HSYA group, medium-dose HSYA group and high-dose HSYA group were higher than those of the sham operation group (all P<0.05).The levels of 3-NT, iNOS and NO in the brain tissues of rats in the low-dose HSYA group were not significantly different from those in the model group (P>0.05).The levels of 3-NT, iNOS, NO in the medium-dose and high-dose HSYA groups were lower than those in the model group and low-dose HSYA group (all P<0.05), and the levels of 3-NT, iNOS and NO in brain tissues of high-dose HSYA group were lower than those in medium-dose HSYA group (all P<0.05).Conclusion HSYA can inhibit the expression of 3-NT, iNOS, and NO in rat brain tissues after ischemia-reperfusion.The higher the HSYA dose is, the more obvious the effect will be.关键词
羟基红花黄色素A/脑/缺血再灌注损伤/3-硝基酪氨酸/过氧化亚硝基阴离子/一氧化氮合成酶/一氧化氮Key words
hydroxysafflor yellow A/brain/ischemia-reperfusion injury/3-nitrotyrosine/peroxynitrite/nitric oxide synthase/nitric oxide/animal experiments分类
医药卫生引用本文复制引用
肖沉,孙莉,曹杉杉,徐艳炜,梁浩,程焱..尾静脉注射不同剂量羟基红花黄色素A的缺血再灌注大鼠脑组织3-NT、iNOS、NO表达观察[J].山东医药,2017,57(11):15-18,4.基金项目
国家自然科学基金资助项目(81571201,81401023) （81571201,81401023）
天津市科委重点项目(13ZCZDSY01900). （13ZCZDSY01900）
