上海海洋大学学报2017，Vol.26Issue(2)：294-300,7.DOI:10.12024/jsou.20160401731
超高压对单增李斯特菌生物被膜形成的影响
Effects of ultra-high pressure (UHP) processing on biofilm formation of Listeria monocytogenes
摘要
Abstract
We found Listeria monocytogenes was still alive after the ultra-high pressure processing.Based on the result of our former experiments,this research aims to investigate whether the ultra-high pressure processing affects the biofilm formation of L.monocytogenes.2 strains of L.monocytogenes were applied in the research,both serotypes were 4b.Crystal violet staining assay was used to investigate the biofilm forming capacity of 2 strains,while the fluorescent microscopy and SEM were applied to observe the structures of the biofilm.The biofilm forming capacity of WaX12 was higher than ATCC13932.After 100 MPa UHP processing,WaX12 formed more biofilm than the control group,while 200 MPa improved the biofilm forming capacity of ATCC13932.The biofilm formation of both strains reduced after 300 MPa and higher UHP processing.The images of biofilm formation of the 2 strains obtained by fluorescent microscopy and SEM were consistent with the results of crystal violet staining assay.Results suggested that the UHP processing may increase the biofilm formation of L.monocytogenes.If the pressure was lower than 200 MPa,the biofilm formation was strengthened,and reached the maximum after 48 h.It is important to choose proper pressure during UHP processing.关键词
超高压/单增李斯特菌/生物被膜/SEMKey words
UHP processing/Listeria monocytogenes/biofilm/SEM分类
生物科学引用本文复制引用
莘似韵,孙晓红,徐忆宁,赵勇,吴启华,潘迎捷..超高压对单增李斯特菌生物被膜形成的影响[J].上海海洋大学学报,2017,26(2):294-300,7.基金项目
上海市科技兴农重点攻关项目(沪农科攻字2014第3-5号) （沪农科攻字2014第3-5号）
上海市科委工程中心能力提升项目(16DZ22803001) （16DZ22803001）
