生命科学研究2017，Vol.21Issue(1)：41-45,54,6.DOI:10.16605/j.cnki.1007-7847.2017.01.008
抑制CYP4A11基因表达的siRNAs优选及对血管收缩相关因子的影响
Optimization of siRNAs in Inhibiting the Expression of CYP4A11 Gene and Effects on Related Factors Affecting Blood Vessel Contraction
摘要
Abstract
To investigate the relationship between the suppressed expression of the metabolic enzyme CYP4A11 in the human cells and the vasoconstriction-related factors,gene silencing techniques were used to suppress CYP4A 11 expression in cells after siRNAs were online designed and verified by BLAST.Three siRNAs were synthesized and transferred into the EA.hy926 cells.After detection by florescence expression and qPCR,and verification by transfection of MG63 cells which could express higher amount of CYP4A11,siRNA2 at a dose of 25 nmol/L proved to have the best inhibitory effect on CYP4A 11.When 25 nmol/L siRNA2 was used to inhibit the expression of CYP4A 11 in EA.hy926 cells,the expressions of vasoconstriction-related factors ET1R and AT1R decreased significantly (P＜0.05) and eNOS was upregulated obviously (P＜0.001),as detected by qPCR and Western-blot.Therefore,siRNA2 can significantly inhibit the expression of CYP4A 11 in EA.hy926 cells,and regulate the related factors that affect the tension in blood vessels,promoting blood vessel relaxation.关键词
CYP4A11/小干扰RNA (siRNA)/血管收缩因子/内皮素1受体(ET1R)/血管紧张素1型受体(AT1R)/内皮型一氧化氮合酶(eNOS)Key words
CYP4A11/small interfering RNA (siRNA)/vascular contraction factor/endothelin 1 receptor (ET1R)/angiotensin type 1 receptor (AT1R)/endothelial nitric oxide synthase (eNOS)分类
生物科学引用本文复制引用
李晋,胡静,李健,石磊,赵树进..抑制CYP4A11基因表达的siRNAs优选及对血管收缩相关因子的影响[J].生命科学研究,2017,21(1):41-45,54,6.基金项目
广州市科技计划项目(201509010012) （201509010012）
