生命科学研究2017，Vol.21Issue(1)：46-50,5.DOI:10.16605/j.cnki.1007-7847.2017.01.009
FeCl3诱导大鼠颈总动脉血栓制备慢性脑缺血模型的探索
FeCl3 Induced Carotid Artery Thrombosis to Build Chronic Cerebral Ischemia Model in Rat
摘要
Abstract
Ligation of bilateral common carotid arteries in rats is usually used to create chronic cerebral ischemia model.But the advantages of making this model by using FeCl3 remain unclear.Here,four model groups of rats were treated with FeCl3 at contents of 20％,35％,50％ and 70％,respectively,and the control group was treated with the ligation method.Mortality rate of rats,neurological function score,HE staining,Y-maze test and the weight of the common carotid artery thrombosis were used to evaluate the model's stability and success rate.The results demonstrated that the death rates of model groups treated with 20％,35％ and 50％ concentrations were 5％,15％,10％,respectively,significantly lower than that of the ligation group (x2=8.533,P=0.003;x2=4.286,P=0.038;x2=6.144,P=0.013).Compared with the sham group,the model groups and ligation group had no significant change (P＞0.05) in neurological function score.HE staining results showed that the concentrations of 35％ and 50％ can form a better model of arterial stenosis.Y-maze training of the model groups (35％,50％) had no significant change compared with the ligation group (P＞0.05).The weights of thrombus from low concentration group were significantly decreased compared with that from the middle/high concentration group (P＜0.01),but there was no significant change between 35％ and 50％ model groups (P＞0.05).These data suggested that this method can improve the success rate and stability of chronic cerebral ischemia model,and that the best choice of concentration is 35％ or 50％.关键词
FeCl3/慢性脑缺血/模型/颈总动脉/大鼠Key words
FeCl3/chronic cerebral ischemia/model/common carotid artery/rat分类
医药卫生引用本文复制引用
梁辰,陈本宏,王福文..FeCl3诱导大鼠颈总动脉血栓制备慢性脑缺血模型的探索[J].生命科学研究,2017,21(1):46-50,5.基金项目
国家科技重大专项课题(2009ZX09301-013) （2009ZX09301-013）
