生命科学研究2017，Vol.21Issue(1)：51-54,4.DOI:10.16605/j.cnki.1007-7847.2017.01.010
房颤患者心房肌细胞的原代培养与应用
Primary Culture and Application of Atrial Myocytes from Patients with Atrial Fibrillation
摘要
Abstract
Exploring the methods for primary culture of atrial myocytes from patients with atrial fibrillation (AF) can provide practical methods for research about AF.Here,the left atrial appendage specimens were obtained from patients with AF during cardiac surgery.Then,primary atrial myocytes,which were isolated from the specimens by collagenase digestion method,were identified through immunohistochemistry and analyzed simultaneously.In contrast with some animal experiments,low expressions of connexin40 (Cx40) and Kv1.5 channel protein were found in AF patients.The results showed that studying atrial myocytes of AF patients will be more reliable.Meanwhile,atrial myocyte culture from AF patients is the basis for research on the pathogenesis of AF.关键词
心房颤动(AF)/心房肌细胞/细胞培养/缝隙连接蛋白40 (Cx40)/钾离子通道(Kv1.5)Key words
atrial fibrillation (AF)/atrial myocytes/cell culture/connexin40 (Cx40)/K+ channel (Kv1.5)分类
医药卫生引用本文复制引用
卞誉豪,张飞..房颤患者心房肌细胞的原代培养与应用[J].生命科学研究,2017,21(1):51-54,4.基金项目
深圳市科创委2013年度知识创新计划-基础研究项目(JCYJ20130322154529556) （JCYJ20130322154529556）
深圳市南山区2012年度科技计划项目(南科研卫2012003号) （南科研卫2012003号）
