生态学报2017，Vol.37Issue(5)：1650-1658,9.DOI:10.5846/stxb201510052000
基于高通量测序的鄱阳湖典型湿地土壤细菌群落特征分析
High throughput sequencing analysis of bacterial communities in soils of a typical Poyang Lake wetland
摘要
Abstract
Bacterial communities are crucial for the functioning of wetlands as they play a critical role in energy flows and nutrient transformation.Poyang Lake,the largest freshwater lake in China,forms a large area of wetland due to its great changes in seasonal water levels,which play an important ecological role in the middle and lower reaches of Yangtze River.In this study,surface soils (0-10 cm) covered with typical plants were sampled at five sites from the lake surface to a sloping field in Poyang Lake wetland in November 2014.Chemical parameters measured in the soil included NH4+-N,NO3--N,TN,TP,and SOC.We profiled the bacterial community structure by sequencing the V3-V4 16S rRNA gene on the Illumina Miseq PE300,using 338F (5'-ACTCCTACGGGAGGCAGCA-3') and 806R (5'-GGACTACHVGGGTWTCTAAT-3') primers.A total of 134,223 bacterial sequences were obtained,and a similarity of 97％ was used to identify operational taxonomic units (OTUs),which resulted in a total of 2,072.The phylogenetic affiliation of each 16S rRNA gene sequence was analyzed by an RDP Classifier against the Silva 16S rRNA database.The coverage of all soils was over 99％,indicating a deep sequencing.The bacterial community richness calculated by the Chao1 estimator and the bacterial community diversity calculated by the Shannon index showed the same trend among the five soils covered with different plants:Carex soil ＞ Carex-Phalaris soil ＞ Phragmites soil ＞ mud flat soil ＞ Artemisia soil.The principal component analysis of OTUs showed that the bacterial community structure was more similar among soils that were closer along the transition from the lake surface to the sloping field.The bacterial community structure was similar among Carex-Phalaris soil,Carex soil,and Phragmites soil,but very different between mud flat soil and Artemisia soil.Proteobacteria (30.0％ of total sequences) was the most abundant phylum in Poyang Lake wetland,mainly including Alphaproteobacteria (7.2％),Betaproteobacteria (8.9％),Deltaproteobacteria (10.5％),and Gammaproteobacteria (2.8％).Except for Proteobacteria,other phyla with high abundance were Acidobacteria (16.7％),Chloroflexi (16.5％),Nitrospirae (10.2％),Firmicutes (7.5％),Actinobacteria (4.8％),Gemmatimonadetes (3.8％),and Chlorobi (1.8％).Proteobacteria was the most abundant phylum in Carex,Carex-Phalaris,and Phragmites soils,and the third most abundant phylum in mud flat and Artemisia soils,after Chloroflexi and Nitrospirae (first abundant phylum in mud flat and Artemisia soils,respectively),and Acidobacteria and Chloroflexi (second abundant phylum in mud flat and Artemisia soils,respectively).Most of the phyla's relative abundance had an obvious trend along the transition from the lake surface to the sloping field.Nitrospira (10.2％) was the most abundant genus in this area,and the Nitrospirae abundance was much higher than that in other wetland soils,reflecting the importance of the nitrogen cycle in the ecological system of Poyang Lake wetland.There were many norank or uncultured genera,making it difficult to study their ecological functions.Redundancy analysis of bacterial phyla and chemical parameters in soil showed that TP,NH~-N,and SOC had more relevance to the bacterial community structure.The results of this study showed that there were differences in bacterial community structure among soils covered with different plants in the Poyang Lake wetland,and these differences had regular variation along the transition from the lake surface to the sloping field.关键词
鄱阳湖湿地/高通量测序/细菌多样性/细菌群落结构Key words
Poyang Lake wetland/high throughput sequencing/bacterial diversity/bacterial community structure引用本文复制引用
王鹏,陈波,张华..基于高通量测序的鄱阳湖典型湿地土壤细菌群落特征分析[J].生态学报,2017,37(5):1650-1658,9.基金项目
江西省自然科学基金(20151BAB213035) （20151BAB213035）
鄱阳湖湿地与流域研究教育部重点实验室(江西师范大学)主任开放基金(ZK2013009) （江西师范大学）
江西省教育厅科技计划项目(GJJ14267) （GJJ14267）
江西省重大生态安全问题监控协同创新中心资助项目(JXS-EW-00) （JXS-EW-00）
