山西农业大学学报（自然科学版）2017，Vol.37Issue(3)：153-157,5.
乌拉尔图小麦全基因组NBS-SSR位点分析及标记开发
Genome-wide analysis and marker development of NBS-SSR in Triticum urartu
摘要
Abstract
[Objective]The aim of this study was to diagnose the SSR loci on genome sequences of the NBS family in Triticum urartu and to develop molecular markers for the exploitation and utilization of this resistance resources.[Method]In this paper,the target sequences were getted by searching the database using NBS HMM,and molecular markers were developed based on the SSR loci diagnosed by software,then the markers were verificated by two T.urartu materials which resistant (UR207) and susceptible (UR203) to the Blumeria graminis tritici,respectively.[Results]463 NBS genes,which can be divided into four categories containing N,CN,NL and CNL,were separated from the T.urartu genome.4 292 SSR loci were found on the scaffold sequences with NBS and of which the dinucleotide repeat loci is most,accounting for 78.1％.42 TuNBS-SSR markers distributing in the each chromosome arm were developed randomly and 12 markers showed polymorphism between UR207 and UR203.[Conclusion]4 292 NBSSSR loci were diagnosed from T.urartu genome and 12 markers exhibited polymorphism between resistant and susceptible materials.The results can be used for the identification of disease resistance and the screening of resistance genes of T.urartu.关键词
乌拉尔图小麦/NBS类基因/SSR标记/抗病Key words
T urartu/NBS-encoding genes/SSR markers/Disease resistance分类
农业科技引用本文复制引用
乔麟轶,畅志坚,张晓军,刘静,朱艳,詹海仙,郭慧娟,李欣..乌拉尔图小麦全基因组NBS-SSR位点分析及标记开发[J].山西农业大学学报（自然科学版）,2017,37(3):153-157,5.基金项目
国家自然科学青年基金项目(31601303),山西省青年基金项目(2015021145),山西省农业攻关项目(20150311001-1),山西省农科院攻关项目(15YGG01,YGG1602) （31601303）
