山西农业大学学报（自然科学版）2017，Vol.37Issue(3)：177-182,6.
AtDES1基因在大白菜中的遗传转化
The genetic transformation of Chinese cabbage with DES1 gene
摘要
Abstract
[Objective]Hydrogen sulfide,considered as the third gasotrasmitter after nitric oxide and carbonic oxide,is playing a significant role in plant developmental processes and defenses.In recent years,with the increased environmental pollution,the growth and developmental processes of Chinese cabbage have been seriously inhibited.L-eysteine desulfhydrase (DES1) is an important enzyme to produce hydrogen sulfide.Integrating DES1 into cabbage genome can not only increase its stress resistance,but provide cabbage breeding with new germplasm.[Methods]On the basis of the existed transformation system,this study using ‘zhongbai 60’ as material optimized the seed sterilization time,microbial infection time and antibiotics concentration,so as to acquire an efficient transgenic system.[Results]The results indicated that seeds disinfectived with 0.1％ HgCl2 for 5 min could maintain a higher rate of germination and it could also prevent the formation of endogenous bacteria simultaneously.When the explants were immersed in bacterium concentration of OD600 =0.2～0.3 for 5 min and 7.5 mg·L-1 and 5.0 mg·L-1 were respectively resgarded as the resistant selection pressure for shoots and roots,the transformation rate reached up to 6 ％.[Conclusion]The results of PCR and qRT-PCR turned out that DES1 was successfully integrated into the genome of Chinese cabbage,meanwhile,the expression of DES1 has improved in different degrees.关键词
大白菜/硫化氢/转化/转基因植株Key words
Chinese cabbage/Hydrogen sulfide/Transformation/Transgenic plant分类
生物科学引用本文复制引用
景巧丽,马晓丽,裴雁曦..AtDES1基因在大白菜中的遗传转化[J].山西农业大学学报（自然科学版）,2017,37(3):177-182,6.基金项目
国家自然科学基金(31372085) （31372085）
