山西农业大学学报（自然科学版）2017，Vol.37Issue(3)：189-193,5.
平陆百合愈伤组织诱导的正交试验研究
Callus induction of Lilium brownii var.viridulum by orthogonal test
摘要
Abstract
[Objective] Search the optimal medium of callus inducement of Lilium brownii var.viridulum.[Methods] With the scales of Lilium as explants,the effect of different hormone combines (2,4-D、NAA、KT)on the callus induction of Lilium were studied by design test in light and darkness respectively.[Results]In light,the effect of three hormones on callus induction was NAA＞KT＞2,4-D.The optimal medium for callus induction of Lilium was MS+2,4-D 1 mg·L-1 +NAA 0.3 mg·L-1 +KT 0.5 mg·L-1,the callus induction frequency was 57.143％;In darkness,the effect of three hormones on callus induction was KT＞NAA＞2,4-D.The optimal medium for callus induction of Lilium was MS+ 2,4-D 5 mg· L-1 + NAA 0.3 mg· L-1 + KT 0.2 mg· L-1,the callus induction frequency was 50 ％.[Conclusion]The best medium for callus induction of Lilium brownii var.viridulum was MS+ 2,4-D 1 mg· L-1 + NAA 0.3 mg·L-1 +KT 0.5 mg·L-1,inducing condition was light.关键词
平陆百合/愈伤组织/正交设计Key words
Lilium brownie var.viridulum/Callus/Orthogonal design分类
农业科技引用本文复制引用
任建宏,王鹏丽,殷丽娜,赵娟..平陆百合愈伤组织诱导的正交试验研究[J].山西农业大学学报（自然科学版）,2017,37(3):189-193,5.基金项目
山西省科技攻关项目(20130311014-1) （20130311014-1）
山西农业大学引进人才科研启动项目(2012YJ13) （2012YJ13）
山西省青年科技研究基金(201601D202054) （201601D202054）
