山西农业大学学报（自然科学版）2017，Vol.37Issue(3)：201-206,213,7.
HPLC法分析山西不同大豆品种皂醇含量
Analysis of soyasapogenol contents of different soybean varieties in Shanxi by HPLC method
摘要
Abstract
[Objective]To provide theoretical basis for the selection and mechanism study of soybean varieties with high content of soyasapogenol,the difference of soyasapogenol contents in different grain weight,seed coat color and ecological type of soybean materials were evaluated,soybean genotype materials with high soyasapogenol content were screened.[Methods] The contents of soyasapogenol were determined by high performance liquid chromatography (HPLC) method with 72 soybean varieties from Shanxi as materials.[Results]We found that the soyasapogenol contents of 72 soybean varieties ranged from 0.92 to 4.02 mg·g-1;the contents of soyasapogenol A,B and the total soyasapogenol of small grain materials were significantly higher than those of medium grain and large grain type materials;the soyasapogenol A and total soyasapogenol contents of soybean varieties with black seed coat were significantly higher than those with yellow or green seed coat;the contents of soyasapogenol A and the total soyasapogenol in the wild soybean were significantly higher than those in the cultivated species;In different tissues,the content of soyasapogenol A,B and total soyasapogenol in hypocotyl was the highest,and the content of soyasapogenol in seed coat was the lowest.[Conclusion]The content of soyasapogenol was significantly different in different soybean varieties,the content of soyasapogenol in the variety with the characteristics of small grains,black seed coat,wild materials was the highest.关键词
大豆/皂苷/皂醇/HPLCKey words
Soybean/Saponin/Soyasapogenol/HPLC分类
农业科技引用本文复制引用
王卫东,赵巧玲,高春燕,郭春绒,赵晋忠,杜维俊,岳爱琴..HPLC法分析山西不同大豆品种皂醇含量[J].山西农业大学学报（自然科学版）,2017,37(3):201-206,213,7.基金项目
山西省科技计划项目(20120311005-3) （20120311005-3）
山西省高等学校科技创新项目(2015148) （2015148）
山西省自然科学基金(201601D011076) （201601D011076）
