中国组织工程研究2017，Vol.21Issue(8)：1155-1160,6.DOI:10.3969/j.issn.2095-4344.2017.08.002
整合素α5β1在膝骨关节炎不同退变程度关节软骨中的差异性表达
Differential expression of integrin alpha5beta1 in the degenerated articular cartilage after knee osteoarthritis
摘要
Abstract
BACKGROUND: Integrin α5β1 has been shown to be related to acetabular cartilage degeneration due to developmental dysplasia of the hip.OBJECTIVE: To investigate the expression of integrin α5β1 in differently degenerated cartilage tissues of the knee after knee osteoarthritis.METHODS: Seventy-five degenerated articular cartilage specimens at the weight-bearing surface of femoral lateral and medial condyles were removed from patients with knee osteoarthritis who underwent total knee replacement, and were then allotted to four groups based on Mankin's grading system: normal (n=6), mild degeneration (n=24), moderate degeneration (n=26) and severe degeneration (n=19) groups. The expression of integrin α5β1 in each group was detected using real-time PCR, immunohistochemistry and western blot assay.RESULTS AND CONCLUSION: The detected results were consistent in the three kinds of assays, and the expression level of integrin α5β1 was ranked as follows: severe degeneration group > moderate degeneration group > mild degeneration group > normal group (P < 0.05). To conclude, differential expression levels of integrin α5β1 are found in different degenerative degrees of articular cartilage in knee osteoarthritis. Furthermore, the expression level of integrin α5β1 is increased with the degenerative severity.关键词
组织构建/软骨组织工程/整合素α5β1/骨关节炎/膝骨关节炎/整合素/关节软骨分类
医药卫生引用本文复制引用
刘俊才,陈友霞,李忠,吴绍军,左银龙,李文,何克,张志伟,李仁明..整合素α5β1在膝骨关节炎不同退变程度关节软骨中的差异性表达[J].中国组织工程研究,2017,21(8):1155-1160,6.基金项目
泸州市科技局重点项目(2013-S-48) Funding:the Key Project of the Office of Science and Technology of Luzhou, No. 2013-S-48 （2013-S-48）
