中国组织工程研究2017，Vol.21Issue(8)：1221-1228,8.DOI:10.3969/j.issn.2095-4344.2017.08.013
内皮克隆形成细胞旁分泌对人脐静脉内皮细胞生物学功能的影响
Paracrine effect of endothelial colony-forming cells on biological functions of human umbilical vein endothelial cells
摘要
Abstract
BACKGROUND: Endothelial colony-forming cells (ECFCs) transplantation exerts beneficial impact on angiogenesis of impaired tissues caused by ischemia. However, whether this impact is related to the paracrine effect of ECFCs needs to be studied further.OBJECTIVE: To detect the cytokine secretion profile of human umbilical cord blood derived-ECFCs conditioned medium (ECFCs-CM) and explore the effect of ECFCs-CM on the proliferation, migration and tube formation ability of human umbilical vein endothelial cells (HUVECs).METHODS: Human cord blood derived-ECFCs were isolated, cultured in vitro, and then identified based on the previous studies. The cytokines in serum free ECFCs-CM were detected using a cytokines antibody array. HUVECs were cultured with ECFCs-CM, serum free EBM-2 as control. The proliferation, migration and tube formation abilities of HUVECs were examined by cell counting kit-8, scratch test and Matrigel assay, respectively.RESULTS AND CONCLUSION: The cultured cells demonstrated typical characteristics of ECFCs, which showed cobblestone appearance and were positive for CD34, KDR and CD144, but not CD45 or CD133, uptook Dil-acLDL and bond FITC-UEA-I, and tube-like structures were formed on Matrigel. The cytokine antibody array showed that ECFCs-CM significantly upregulated the expressions of 30 kinds of angiogenic factors. Compared with the control group,the HUVECs cultured with ECFCs-CM showed significantly improved proliferation ability at 24, 48 and 72 hours (P <0.01). The migration rate of HUVECs in the experimental group was significantly higher than that in the control group at 12 and 24 hours (P < 0.01). There were more tubular structures in the experimental group than those in the control group (P < 0.05). These results indicate that ECFCs can promote the bioactivity of mature endothelial cells through paracrine action.关键词
组织构建/内皮细胞/内皮祖细胞/内皮克隆形成细胞/细胞培养/旁分泌/条件培养基/细胞增殖/血管新生/血管生成/慢性创面愈合/国家自然科学基金分类
医药卫生引用本文复制引用
王布霖,郭晓瑞,李勤..内皮克隆形成细胞旁分泌对人脐静脉内皮细胞生物学功能的影响[J].中国组织工程研究,2017,21(8):1221-1228,8.基金项目
国家自然科学基金面上项目(81571910) （81571910）
广东省科技计划项目(2014A020212256) （2014A020212256）
广州市科技计划项目(201607010394) Funding:the National Natural Science Foundation of China, No. 81571910 （201607010394）
the Science and Technology Program of Guangdong Province, No. 2014A020212256 （）
the Science and Technology Program of Guangzhou, No. 201607010394 （）
