PM2.5对小鼠泪膜功能和角膜上皮组织结构的影响
Effects of PM2.s on tear film function and corneal epithelial structure in mice
摘要
Abstract
Objective To investigate the effects of PM2.5 on tear film function and corneal epithelial structure in mice.Methods Totally 24 male BALB/c mice (24 eyes) were divided into two groups:group A (with PBS eye drops,n =12),group B (5 mg · mL-1 PM2.5 eye drop group,n =12).PBS and PM2.5 eye drop were given with four times per day for 7 consecutive days in right eye.Tear secretion level was measured with phenol red thread.Break-up time (BUT) of tear film was tested,and corneal fluorescein staining (FL) was scored before therapy and 1 day,4 days and 7 days after droppings and HE staining was performed 7 days after droppings,respectively.Results There was no significant difference in the tear secretion levels,BUT,FL between the groups A and B before treatment (all P ＞ 0.05).At 4 days,7 days after treatment with PM2.5,the mean differences of the group B showed all items significantly changed compared with those before treatment (all P ＜ 0.05).For the group A,there was no statistical change in tear secretion levels,BUT,FL at 7 days after treatment (all P ＞ 0.05).There were statistical differences in all items between group A and B at each time point (all P ＜ 0.05).At 7 days after therapy,the mean layers of corneal epithelial cells in the group A (4 ± 1) was significantly lower than that in the group B (7 ±l) (P ＜0.05).The group B showed that the whole corneal fluorescein staining obviously increased,and corneal epithelial cell layer was thickened.Conclusion PM2.5 can influence tears film function and damage the corneal epithelial structure in mice.关键词
PM2.5/泪膜功能/角膜上皮/小鼠Key words
PM2.5/tear film function/corneal epithelium/mouse分类
医药卫生引用本文复制引用
李娟,丁小艳,王亚虹,叶鳞泓,叶蕾,姜楠,邵毅..PM2.5对小鼠泪膜功能和角膜上皮组织结构的影响[J].眼科新进展,2017,37(3):201-204,4.基金项目
国家自然科学基金资助(编号:81160118、81400372、81400424、81660158) （编号:81160118、81400372、81400424、81660158）
陕西省科学技术研究发展计划项目(编号:2014K11-03-07-04) （编号:2014K11-03-07-04）
2017年陕西省创新人才推进计划项目 （）
江西省远航工程(编号:2014022) （编号:2014022）
江西省自然科学基金重大项目(编号:2016ACB21017) （编号:2016ACB21017）
江西省青年科学基金(编号:20151BAB215016) （编号:20151BAB215016）
江西省科技支撑计划项目(编号:20151BBG70223)National Natural Science Foundation of China (No:81160118,81400372,81400424,81660158) （编号:20151BBG70223）
Science and Technology Research and Development Project of Shaanxi Province (No:2014K11-03-07-04) （No:2014K11-03-07-04）
Innovative Talents Promotion Project of Shaanxi Province(2017) （2017）
Jiangxi Province Voyage Project (No:2014022) （No:2014022）
Natural Science Key Project of Jiangxi Province (No:20161ACB21017) （No:20161ACB21017）
Youth Science Foundation of Jiangxi Province (No:20151BAB215016) （No:20151BAB215016）
Technology and Science Foundation of Jiangxi Province (No:20151BBG70223) （No:20151BBG70223）
