眼科新进展2017，Vol.37Issue(3)：210-214,5.DOI:10.13389/j.cnki.rao.2017.0054
针刺对干眼兔泪腺形态学及泪腺上皮细胞凋亡相关基因蛋白表达的影响
Effects of acupuncture on morphology and apoptosis related gene expression of lacrimal gland in dry eye model rabbits
摘要
Abstract
Objective To investigate the effects of acupuncture on morphology and apoptosis related gene expression of lacrimal gland in dry eye model rabbit,and explore the possible mechanism of acupuncture on dry eye.Methods Eighteen healthy New Zealand white rabbits were randomly divided into three groups:blank group,model group,acupuncture group,6 rabbits in each group.Blank group did not accept any treatment.Model group and acupuncture group were treated with subcutaneous injections of scopolamine hydrobromide,4 times a day with the dose of 2.0 mg · kg-1,when the acupuncture group was also treated with acupuncture,once a day for fourteen days.Three groups were examined Schirmer Ⅰ test (S Ⅰt) at different stages,and morphological changes of lacrimal gland under light and electron microscope were observed.The immunohistochemical method was used to detect the protein levels of apoptosis related gene in lacrimal gland of all rabbits.Results Between the acupuncture group and model group,the S Ⅰt was significantly different on day 4,7,14 (all P ＜ 0.05).At the same time,compared with that before experiment,S Ⅰt had no significant difference on day 14 in acupuncture group (P ＞ 0.05).Optical and electron microscope showed the lacrimal gland epithelial cells of acupuncture group closely arranged with abundant cytoplasm,closed to that of the blank group.Meanwhile,the number of lacrimal epithelium cells with Bax,Fas and FasL positive expression was significantly less than that in the model group (all P ＜ 0.05).But when it compared with blank group,there was no significant difference of Bax/Bcl-2 and Fas/FasL positive expression in acupuncture group (all P ＞ 0.05).Conclusion Acupuncture can restrain the apoptosis and gland atrophy of lacrimal gland tissue of dry eye rabbit,and promote metabolism,thereby increase the tear secretion.关键词
针刺/干眼/泪腺/细胞凋亡Key words
acupuncture/dry eye/lacrimal gland/cell apoptosis分类
医药卫生引用本文复制引用
陈星如,高卫萍..针刺对干眼兔泪腺形态学及泪腺上皮细胞凋亡相关基因蛋白表达的影响[J].眼科新进展,2017,37(3):210-214,5.基金项目
国家自然科学基金资助(编号:81373746)National Natural Science Foundation of China (No:81373746) （编号:81373746）
