眼科新进展2017，Vol.37Issue(3)：215-219,5.DOI:10.13389/j.cnki.rao.2017.0055
雌二醇对视网膜缺血-再灌注损伤后基质细胞衍生因子-1表达的影响
Effects of 17β-estradiol on stromal cell-derived factor-1 expression after retinal ischemia-reperfusion injury
摘要
Abstract
Objective To examine the expression of stromal cell-derived factor-1 (SDF-1) in a rat model of retinal ischemia-reperfusion injury (RIRI),and investigate the protective effect of 17β-Estradiol (E2) on RIRI and explore the mechanism.Methods The RIRI model was established in Sprague-Dawley rats by increasing the intraocular pressure.Relative expression levels of SDF-1 mRNA and protein in the retina at 6 hours,12 hours and 24 hours following reperfusion was determined by RT-PCR and Western blot,respectively.E2 was administered to investigate the effects of estrogen on SDF-1 expression,and the estrogen receptor antagonist ICI 182-780 was administered to investigate the effect of estrogen receptor on the expression of SDF-1.Results SDF-1 expression in RIRI 6 hours group,12 hours group and 24 hours group was increased compared with normal control group (all P ＜ 0.05),with maximum expression at RIRI 12 hours group.As expected,pretreatment of RIRI rats with E2 had a protection on RIRI retina;SDF-1 expression was increased in RIRI + E2 group compared with IR control group and RIRI + vehicle group (all P ＜ 0.05).RIRI + E2 + ICI 182-780 group could decrease SDF-1 expression compared with RIRI + E2 group(all P ＜ 0.05).Conclusion E2 offers protection against RIRI by inducing an up-regulation in SDF-1 expression through activation of the estrogen receptor.关键词
视网膜/缺血-再灌注损伤/雌二醇/基质细胞衍生因子-1Key words
retina/ischemia-reperfusion/17β-estradiol/stromal cell-derived factor-1分类
医药卫生引用本文复制引用
王业青,张晓梅,董丽,石焕琦,毕文娇,侯文文..雌二醇对视网膜缺血-再灌注损伤后基质细胞衍生因子-1表达的影响[J].眼科新进展,2017,37(3):215-219,5.基金项目
黑龙江省卫生计生委科研课题(编号:2014-299) （编号:2014-299）
黑龙江省博士后科研启动金(编号:LBH-Q15101)Health Development Planning Commission Science Foundation of Heilongjiang Province (No:2014-299) （编号:LBH-Q15101）
Postdoctoral Scientific Research Foundation of Heilongjiang Province (No:LBH-Q15101) （No:LBH-Q15101）
