中国临床药理学杂志2017，Vol.33Issue(3)：239-243,5.DOI:10.13699/j.cnki.1001-6821.2017.03.014
红芪多糖对db/db小鼠糖尿病心肌病心肌纤维化的改善作用
Improvement of Hedysarum Polybotrys Polysacchcaide on myocardial fibrosis in db/db mice with diabetic cardiomyopathy
摘要
Abstract
Objective To explore protection mechanism of Hedysari polysaccharide (HPS) on myocardial fibrosis of db/db mice with diabetic cardiomyopathy.Methods Sixty db/db mice (7-week-old) were randomly divided into five groups:high-dose experimental group,middle-dose experimental group,low-dose experimental group (HPS:200,100,50 mg · kg-1),control group(rosiglitazone:4 mg · kg-1) and model group(0.9％ NaCl,gavege);while 12 db/m mice (7-week-old) were normal group.Each group had 12 mice.Blood glucose was detected at the baseline before intervention and at end of 2,4,6 and 8 weeks after intervention separately.The activity of blood lipid,superoxide dismutase (SOD),glutathione peroxidase (GSH-Px) and the content of malondialdehyde (MDA) were detected by biochemical method after 8 weeks.The degree of myocardial fibrosis was observed by masson staining.The qPCR method and Western blotting method were used to detect the expression of peroxisome proliferator-activated receptor γ (PPARγ),nuclear transcription factor 2 kappa B (NF-κB) mRNA in myocardial tissue.Results The activities of SOD(mg · mL-1)in model group,high-dose experimental group,middle-dose experimental group,control group were 140.70 ± 1.04,145.81 ± 0.99,142.21 ± 1.09,145.70 ± 1.10,respectively with statistical difference compared with the model (P ＜ 0.05).The activities of GSH-PX(U ·mg-1) in above four groups were 110.91 ±0.82,114.94-0.78,112.10 ±0.86,114.84 ±0.86 with statistical difference compared with the model(all P ＜0.01).The contents of MDA(nmol · mg-1) were 7.20 ±0.49,5.82 ±0.52,6.62 ±0.67,5.80 ±0.52 in the four groups which significantly decreased (P ＜ 0.05,P ＜ 0.01).The expression of PPARγ mRNA were 0.34 ± 0.11,0.68 ± 0.05,0.48 ±0.03,0.49 ±0.03,0.93 ± 0.05 and protein above were 0.75 ±0.12,1.78 ±0.08,1.44 ±0.07,1.07 ±0.05,1.63 ±0.02 in above five groups with statistical difference compared with the model(all P ＜0.05).NF-kappa B mRNA were 3.35 ±0.81,1.67 ±0.22,2.34 ±0.39,2.05 ±0.44 and protein were 1.17 ±0.04,0.56 ±0.12,0.86 ±0.13,0.55± 0.12 in the four groups with statistical difference compared with the model (all P ＜ 0.05).Conclusion HPS can reduce the development of myocardial fibrosis by increasing the expression of PPARγ and inhibiting the activity of NF-κB,controlling oxidative stress and inflammatory reaction through the PPARγ-NF-κB signaling pathway.关键词
糖尿病心肌病/红芪多糖/db/db小鼠/超氧化物歧化酶/丙二醛/过氧化物酶体增殖物激活受体γ/核转录因子kappa BKey words
diabetic cardiomyopathy/Hedysarum Polybotrys Polysacchcaide/db/db mice/superoxide dismutase/malonaldehyde/peroxisome proliferator-activated receptor γ/nuclear transcription factor-kappa B分类
医药卫生引用本文复制引用
张花治,金智生,王东旭,和彩玲,王栋,谢卓霖,楚惠媛,何流..红芪多糖对db/db小鼠糖尿病心肌病心肌纤维化的改善作用[J].中国临床药理学杂志,2017,33(3):239-243,5.基金项目
国家自然科学基金地区科学基金资助项目(81360538) （81360538）
甘肃省青年科技基金计划基金资助项目(145RJYA297) （145RJYA297）
