中国临床药理学杂志2017，Vol.33Issue(3)：248-250,3.DOI:10.13699/j.cnki.1001-6821.2017.03.016
含碘造影剂对人肾小管上皮细胞坏死的诱导作用
Induce effect of iodinated contrast media on necrosis of human renal tubular epithelial cell
摘要
Abstract
Objective To explore the effection necrosis of human renal tubular epithelial (HK-2) cell induced by iodinated contrast media.Methods The HK-2 cell was cultured in vitro.The experiment was divided into three groups:control group,iohexol group,urografin group.HK-2 cell was treated with iohexol(75 mgI · mL-1) and urografin(75 mgI · mL-1).Cell vitality was determined by MrTF assay at time of 2 h after intervention and of 5,22 h after drug removal.The release of lactate dehydrogenase (LDH) in liquid supernatant was detected by using LDH kit.Quantitative analysis of Hoechst33342/PI staining results was carried out by high content imaging system.Apoptosis rate and death rate were calculated.Results Compared with the control group,iohexol and urografin induced the cell vitality decreased to (52.98 ± 6.75)％,(47.16 ±4.18)％ at time of 2 h after intervention with statistically significant (P ＜ 0.01).And the release of LDH increased to (201.31 ± 11.69),(256.21 ± 19.82) U · L-1 with statistically significant (P ＜0.01).This effect still existed at 5 h and 22 h after the drugs removed.The apoptosis rates of iohexol and urografin were (23.09 ± 4.25) ％,(38.64 ± 8.32) ％;the necrosis rates of iohexol and urografin were(19.69 ± 2.13) ％,(24.07 ± 6.18) ％,which increased significantly compared with the control group (P ＜ 0.01).Moreover,urografin induced HK-2 cell necrosis more significantly (P ＜ 0.05).Conclusion Iodinated contrast media can induce necrosis of human renal tubular epithelial cell.关键词
造影剂/肾小管上皮细胞/坏死/凋亡Key words
contrast media/renal tubular epithelial cell/necrosis/apoptosis分类
医药卫生引用本文复制引用
关晓凤,陈清洁,尹文俊,李岱阳,汪江林,左笑丛..含碘造影剂对人肾小管上皮细胞坏死的诱导作用[J].中国临床药理学杂志,2017,33(3):248-250,3.基金项目
教育部新世纪优秀人才支持计划基金资助项目(NCET-13-0605) （NCET-13-0605）
中南大学研究生自主探索创新基金资助项目(2016zzts569) （2016zzts569）
