中国农业大学学报2017，Vol.22Issue(3)：6-11,6.DOI:10.11841/j.issn.1007-4333.2017.03.02
玉米南方锈病和普通锈病分子检测技术研究
Molecular detection of Puccinia polysora Underw.and P.sorghi Schw.
摘要
Abstract
The aim of this study was to develop molecular detection methods for rapid identification of corn rust pathogens,Puccinia polysora and P.sorghi,early warning and effective prevention of diseases caused.The DNA regions of rDNA-ITS of isolates,which were collected from Hainan,Henan and Jilin Provinces,were cloned and analyzed (GenBank accession number:HM452902,HQ154021-HQ154038) by using fungal IT Suniversal primers.Specific primers for the detection of P.po/ysora Underw.and P.sorghi Schw.were designed and tested accordingly.The primers showed high detection specificity and sensitivity for P.polysora Underw.and P.sorghi Schw.,respectively.The lowest pathogen DNA content can be detected in common PCR system was 100 and 1 pg/μL,respectively.Rapid detection technology developed in this study will benefit early prediction and effective control of corn rust.关键词
玉米/玉米南方锈病/玉米普通锈病/分子检测Key words
corn/southern corn rust/common corn rust/molecular detection分类
农业科技引用本文复制引用
邢国珍,魏馨,李晶晶,李闯,刘娜,蒋士君,李春奇,郑文明..玉米南方锈病和普通锈病分子检测技术研究[J].中国农业大学学报,2017,22(3):6-11,6.基金项目
河南省科技创新杰出人才计划(154200510024) （154200510024）
河南省重大公益性科研项目(08004) （08004）
