中国全科医学2017，Vol.20Issue(12)：1439-1443,5.DOI:10.3969/j.issn.1007-9572.2017.12.007
双相障碍患者的多巴胺转运受体基因多态性和静息态功能磁共振成像与脑奖赏环路的相关性研究
Correlation Research between the DAT Gene Polymorphism,Resting - State Functional MRI and in Brain Reward Circuits of Patients with Bipolar Disorder
摘要
Abstract
Background With the rapid improvement of medical technology,resting - state functional magnetic resonance (rs - fMRI)technology has been widely used in the clinical research of bipolar disorder (BD). Objective To discuss the correlation between the 40 bp VNTR polymorphism of serum dopamine transporter (DAT)gene,rs - fMRI and human brain reward circuits among patients with BD. Methods According to the inclusion and exclusion criteria,90 BD patients (aged from 20 to 50,all genders,right - handed),hospitalized in Sir Run Run Shaw Hospital,School of Medicine,Zhejiang University from July 2014 to July 2015 and signed the informed consent,were selected in the study. According to the staging of disease,patients were divided into manic group of 32 patients with BD (group A),depressive group of 31 patients with BD (group B),remission phase group of 27 patients with BD (group C),and control group of 30 healthy persons during the same period (group D). The patients in the 3 groups were conducted with functional magnetic resonance imaging (fMRI),and then rs - fMRI was analyzed by the NIH - developed data processing platform of brain function. The regions of increased and decreased ReHo were investigated by targeting at different neural features. Polymerase chain reaction - restriction fragment length polymorphism (PCR - RFLP)technique was used to detect the polymorphism of DAT gene 40 bp VNTR in peripheral blood of the four groups. The polymorphism of this locus and the association of rs - fMRI with human brain reward circuits were analyzed. Results The significantly increased ReHo values of rs - fMRI in group A were mainly distributed at the ventral striatum,and the right anterior cingulate gyrus,while the significantly decreased ReHo values were distributed at the bilateral precuneus regions. The significantly increased ReHo values were distributed at the right insular lobe cortex,left middle frontal gyrus,left precuneus,the significantly decreased ReHo values in group B were distributed at the right anterior lobe of cerebellum,right precentral gyrus,etc;and the significantly increased ReHo values in group C were mainly distributed at the ventral striatum,right anterior cingulated gyrus,bilateral hippocampal,while the significantly decreased ReHo values were distributed at the pons,left postcentral gyrus,central,left inferior frontal gyrus and right cingulate gyrus. There was significant difference in the overall distribution of the genotype frequency of DAT gene 40 bp VNTR loci among the subjects in the 4 groups (P > 0. 10). There was no significant difference in the heterozygosity distribution in the 4 groups (P > 0. 10). Conclusion BD is associated with the abnormal interaction of the related brain regions of brain reward circuits,but their molecular mechanism needs further research and exploration.关键词
双相情感障碍/受体,多巴胺/奖赏环路Key words
Bipolar disorder/Receptors/dopamine/Reward circuits分类
医药卫生引用本文复制引用
王豪杰,陈炜,覃艳华,李翔,周妙平..双相障碍患者的多巴胺转运受体基因多态性和静息态功能磁共振成像与脑奖赏环路的相关性研究[J].中国全科医学,2017,20(12):1439-1443,5.基金项目
国家自然科学基金资助项目(81371490) （81371490）
