中国全科医学2017，Vol.20Issue(12)：1449-1456,8.DOI:10.3969/j.issn.1007-9572.2017.12.009
不同频率电针治疗阿尔茨海默病大鼠神经性病变的机制研究
Mechanism of Different Frequencies of Electroacupuncture in Treating Neuropathy in Rats with Alzheimer′s Disease
摘要
Abstract
Objective To explore the mechanism of different frequencies of eletroacupuncture in the treatment of neuropathy in Alzheimer′ s disease (AD ) rats,so as to provide a theoretical basis for clinical treatment of AD with electroacupuncture. Methods This study was conducted between March and June 2016. Ninety healthy male Wistar rats (SPF) were selected and qualified rats were screened by Morris water maze after 1 week of adaptive feeding. Then the qualified rats were divided into normal group,sham operation group,model group,2 Hz,30 Hz and 50 Hz electroacupuncture treatment groups according to the random number table method with 15 in each. The normal group was not given any treatment. The AD rats were established by injecting Aβ1 - 42 oligomer into the lateral cerebral ventricle in model group,2 Hz,30 Hz and 50 Hz electroacupuncture treatment groups,whereas the rats in the sham operation group were constructed with 0. 9% sodium chloride solution. And the success of modeling was confirmed by Morris water maze test again. After that, the rats in 2 Hz electroacupuncture treatment group were treated by G6805 - Ⅱ type electroacupuncture instrument stimulation at Baihui and Shenshu acupoints with the frequency of 2 Hz. The rats in the 30 Hz and 50 Hz electroacupuncture treatment groups received the same treatment with the same parameters and operations as the 2 Hz electroacupuncture treatment group received except for electroacupuncture frequencies,30 Hz was selected for the 30 Hz electroacupuncture treatment group,and 50 Hz for the 50 Hz electroacupuncture treatment group. The rats in normal group,model group and sham operation group were grabbed in the same way as those rats in the electroacupuncture treatment groups but were put back without any treatment. The rats were sacrificed after intervention and samples of brain hippocampus,cortical tissues were collected. The expression levels of Tau protein,levels of phosphorylated Tau protein at serine 262,369,404 sites 〔Tau (pS262),Tau (pS396),Tau (pS404)〕,14 - 3 - 3ζ and AMPA receptor protein in hippocampus and cortical tissue of rats were detected by Western blotting. Results Compared with the normal group,the expression levels of Tau protein in hippocampus tissue of AD rats in model group significantly decreased (P <0. 05),whereas the levels of Tau (pS262)and Tau (pS396)increased obviously (P < 0. 05). The 50 Hz electroacupuncture treatment group had higher expression level of Tau protein but lower expression levels of Tau (pS262)and Tau (pS396)in the hippocampus tissue than model group,2 Hz and 30 Hz electroacupuncture treatment groups (P < 0. 05). Compared with the normal group,the model group had lower expression level of Tau protein and higher expression levels of Tau (pS262)and Tau (pS404)in the cerebral cortex (P < 0. 05). The 50 Hz electroacupuncture treatment group had higher expression level of Tau protein whereas lower expression level of Tau (pS404 ) in the cerebral cortex than the model group,2 Hz and 30 Hz electroacupuncture treatment groups (P < 0. 05). The expression levels of 14 - 3 - 3ζ in the hippocampus and cortex of the model group were higher than those in the normal group,whereas the expression level of AMPA receptor protein in hippocampus was lower than that in the normal group (P < 0. 05). The 50 Hz electroacupuncture treatment group had lower expression levels of 14 - 3 - 3ζ in hippocampus and cortex but higher expression level of AMPA receptor protein in hippocampus than the model group,2 Hz and 30 Hz electroacupuncture treatment groups (P < 0. 05 ) . Conclusion Different frequencies of electroacupuncture treatment probably improved the neuropathy of AD rats by regulating the expression levels of Tau protein and phosphorylated Tau protein and related proteins (14 - 3 - 3ζ and AMPA),and thus achieved the effect of the treatment of AD.关键词
阿尔茨海默病/电针/Tau蛋白质类Key words
Alzheimer′s disease/Electroacupuncture/Tau proteins分类
医药卫生引用本文复制引用
高珊,孔立红,余超超,姚国晋..不同频率电针治疗阿尔茨海默病大鼠神经性病变的机制研究[J].中国全科医学,2017,20(12):1449-1456,8.基金项目
国家自然科学基金资助项目(81373741)——电针不同频率调控糖原合酶激酶3β对阿尔滋海默大鼠神经突触可塑性影响及机制的研究 （81373741）
