中国全科医学2017，Vol.20Issue(12)：1457-1462,6.DOI:10.3969/j.issn.1007-9572.2017.12.010
白介素18在颞下颌关节炎中的作用研究
Role for IL-18 in Temporomandibular Joint Arthritis
摘要
Abstract
Objective To explore the role of interleukin - 18 (IL-18)in temporomandibular joint arthritis. Methods This study was conducted from November 2015 to April 2016. After being adaptively fed for 1 week,30 male BALB/ c mice (SPF grade)were randomized into 5 groups (group A,group B,group C,group D and group E)with 6 mice in each. Group B, group C,group D and group E were injected collagenase Ⅱ on the 1st and 21st days of intervention,respectively. From the 1st to the 24th days of intervention,group A and group B were injected 0. 9% sodium chloride solution,group C,group D and group E were injected IL-18,IL-18 binding protein (IL-18BP),IL-18 and IL-18BP,respectively. On the 25th day of intervention, peripheral blood and temporomandibular joint lavage fluid of mice were collected. The positive expression rates of the interleukin -18 receptor (IL-18R)in monocytes in peripheral blood and macrophages in temporomandibular joint lavage fluid were detected by flow cytometry (FCM). The levels of TNF-α and IL-1β in peripheral plasma/ liquid supernatant of temporomandibular joint lavage fluid were detected by enzyme - linked immunosorbent assay (ELISA). Results The positive expression rate of IL-18R in peripheral blood monocytes in group B and group C was significantly higher than that in group A (P < 0. 05);the positive expression rate of IL-18R in peripheral blood monocytes in group C was significantly higher than that in group B,group D and group E (P < 0. 05). The positive expression rate of IL-18R in macrophages in temporomandibular joint lavage fluid in group B, group C,group D and group E was higher than that in group A (P < 0. 05);the positive expression rate of IL-18R in macrophages in temporomandibular joint lavage fluid in group C was higher than that in group B,group D and group E (P <0. 05). The levels of TNF-α and IL-1β in peripheral plasma in group B,group C,group D and group E were higher than those in group A (P < 0. 05);the levels of TNF-α and IL-1β in peripheral plasma in group C were higher than those in group B,group D and group E (P < 0. 05). The levels of TNF-α and IL-1β in liquid supernatant of temporomandibular joint lavage fluid in group B,group C,group D and group E were higher than those in group A (P < 0. 05);the levels of TNF-α and IL-1β in liquid supernatant of temporomandibular joint lavage fluid in group C were higher than those in group B,group D and group E (P <0. 05);the level of TNF-α in liquid supernatant of temporomandibular joint lavage fluid in group D was lower than that in group B (P < 0. 05);the level of TNF-α in liquid supernatant of temporomandibular joint lavage fluid in group E was higher than that in group D (P < 0. 05). Conclusion IL-18 can produce pro - inflammatory cytokines such as TNF-α and IL-1β which may play an important role in the occurrence and development of temporomandibular joint arthritis through IL-18R stimulating monocytes/macrophages. IL-18R may be a novel therapy target for temporomandibular joint arthritis.关键词
颞下颌关节障碍/关节炎/白细胞介素18Key words
Temporomandibular joint disorders/Arthritis/Interleukin - 18分类
医药卫生引用本文复制引用
黄芳,胡雅琳,姜程今,刘婧芳,何韶衡,李志刚..白介素18在颞下颌关节炎中的作用研究[J].中国全科医学,2017,20(12):1457-1462,6.基金项目
国家自然科学基金资助项目(81471592) （81471592）
