中国人兽共患病学报2017，Vol.33Issue(1)：15-21,7.DOI:10.3969/j.issn.1002-2694.2017.01.003
鸭源弯曲菌的分离鉴定及其耐药性、毒力基因分析
Isolation, antimicrobial resistance profile and virulence gene analysis of Campylobacter spp.originated from duck
摘要
Abstract
To investigate the contamination conditions of Campylobacter spp.in duck production chain and its antimicrobial resistance,virulence gene distribution,samples were collected at the duck slaughterhouse according to GB 4789.9-2014.Triplex PCR assay was applied to identify the species of Campylobacter and the recovered Campylobacter strains were tested for the antimicrobial susceptibility against 8 kinds of antimicrobial agents using a broth microdilution method,the susceptibility results were determined according to the NARMS criteria (2011).Subsequently,4 virulence genes were detected by PCR method.The result showed that 187 Campylobacter isolates were obtained from 489 samples,including 160 C.jejuni,130 C.coli and 10 unidentified Campylobacter isolates.The total isolation rate of Campylobacter was 38.24％.The prevalence of Camnpytobacter before slaughtering,at depilation stage,evisceration stage and duck products was 76.33％,5.62％,24.00％,and 0％,respectively.The Campylobacter isolates were most frequently resistant to tetracycline (95.72 ％),followed by resistance to clindamycin(90.91％),the resistance rate of azithromycin (63.64％) was in the middle,the resistance rates of ciprofloxacin(31.02％),gentamicin(34.76％),nalidixic acid (37.43 ％),erythromycin (41.18 ％),chloramphenicol (41.18％) were relatively low.The multi-drug resistance was common among Campylobacter isolates with a rate of 72.19％.The prevalence of adhesion-associated gene cadF,flagellin gene flaA,invasion associated protein gene iamA,toxin regulation gene cdtB was 100％,80.75 ％,71.12％ and 94.65％,respectively.The results indicated that the Campylobacter contamination occurred in the slaughtering procedures of duck,and the antimicrobial resistance of Campylobacter isolates was relatively serious.In addition,the virulence-associated genes were detected widely among Campylobacter isolates.Therefore,the supervision of antimicrobial agents using at rearing stage should be strengthened,along with health management in duck production chain.关键词
肉鸭屠宰链/空肠弯曲菌/结肠弯曲菌/耐药性/毒力基因Key words
duck slaughter chain/Campylobacter jejuni/Campylobacter coli/antimicrobial resistance/virulence genes分类
农业科技引用本文复制引用
曾杭,刘书亮,彭峻烽,黄静,严唯玮,陈朋,周康,邹立扣,黄勇,韩新锋..鸭源弯曲菌的分离鉴定及其耐药性、毒力基因分析[J].中国人兽共患病学报,2017,33(1):15-21,7.基金项目
Supported by the grants from the National Natural Science Foundation of China (No.31400066) and the Special Fund for Agroscientific Research in the Public Interest (No.200903055)国家自然科学基金项目(No.31400066)、公益性行业(农业)科研专项子课题(No.200903055)联合资助 （No.31400066）
