中国人兽共患病学报2017，Vol.33Issue(1)：38-42,5.DOI:10.3969/j.issn.1002-2694.2017.01.007
下呼吸道感染来源铜绿假单胞菌毒力基因与氟喹诺酮类药物的耐药性研究
Correlation between virulence genotype and fluoroquinolone drugs resistance in Pseudomonas aeruginosa of lower respiratory tract infection
摘要
Abstract
We investigated the correlation between toxin gene exoS,exoU and fluoroquinolone resistance in lower respiratory tract infection with P.aeruginosa so as to provide guidance for reasonable treatment of clinical infections.We collected P.aeruginosa of sputum samples in hospitalized patients from October 2015 to March 2016.The antimicrobial susceptibility was tested by liquid dilution method.The exoS and exoU genes were detected by PCR technique.Results showed that forty-six P.aeruginosa strains were identified from sputum.The exoS and exoU gene positive rate were 86.96 ％ (40/46) and 69.57 ％ (32/ 46) respectively,and the highest proportion of genotype was exoS+/exoU+ (60.87％,28/46).Among them,36.96％ (17/ 46) were multiple drug-resistant bacteria(MDR).Fluoroquinolone non-sensitive (FQ-NS) strain were 78.95％ (15/19) for MDR and 89.47 ％ (17/19) exoU gene were positive,which was significantly higher than the fluoroquinolone sensitive strains (FQ-S).Compared with the FQ-S strain,FQ-NS strains were serious drug resistance.The drug resistant rate of eefepime and aztreonam were more than 70％,and then meropenem and imipenem were more than 50％.The drugs of lower resistance rate in FQ-NS strain had polymyxin B(10.53％,2/19),amikacin(10.53％,2/19),ceftazidime (15.79％,3/19) and gentamicin (21.05％,4/19).P.aeruginosa of lower respiratory infection carried toxin genes exoS and exoU were higher,the main genetpy was exoS+/exoU+.FQ-NS strains were higher drug resistance rate and a higher proportion of exoU+ strains than FQ-S strains.We should strengthen virulence genes test and drug resistance monitoring in clinical practice.关键词
铜绿假单胞菌/exoS基因/exoU基因/耐药性/氟喹诺酮耐药Key words
Pseudomonas aeruginos/exoS/exoU/resistance drug/fluoroquinolone resistance分类
医药卫生引用本文复制引用
鞠晓红,李瑶,王月华,赵蓬波..下呼吸道感染来源铜绿假单胞菌毒力基因与氟喹诺酮类药物的耐药性研究[J].中国人兽共患病学报,2017,33(1):38-42,5.基金项目
Supported by the Jiangsu Key Laboratory of Medical Science and Laboratory Medicine(No.JSKLM-2014-016)江苏省检验医学重点实验室开放基金项目(No.JSKLM-2014-016) （No.JSKLM-2014-016）
