中国人兽共患病学报2017，Vol.33Issue(1)：43-48,6.DOI:10.3969/j.issn.1002-2694.2017.01.008
基因芯片技术检测结核分枝杆菌利福平和异烟肼耐药性临床应用评价
Evaluation for clinical application effect of gene chip for detection of rifampin and isoniazid resistance in Mycobacterium tuberculosis
摘要
Abstract
We evaluated clinical application effect of gene chip for detection of rifampin (RFP) and isoniazid (INH) resistance in Mycobacterium tuberculosis (MTB).Rifampin and isoniazid drug-resistance gene loci were detected by gene chip with sputum specimens from smear-positive tuberculosis patients and clinical strains,comparing the results of detection.BACTEC MGIT 960 drug susceptibility test results were used as control to evaluate the detection performance of gene chip.The sequences of the polymerase chain reaction products of the rpoB,katG and inhA genes from 999 strains identified as Mycobacterium tuberculosis were determined to confirm the mutations by DNA sequencing.Results showed that 100 cases were identified as nontuberculous mycobacteria by gene chip in the 1 108 cases of smear-positive samples.Among the rest 1 008 samples,there were only 9 cases of microarray results different from BACTEC MGIT960 culture-positive strains,achieving the coincidences of 99.1％.Compared with BACTEC MGIT 960 drug susceptibility test results,the gene chip method displayed a concordance of 98.1 ％ and 94.5 ％ for RFP and INH respectively in the 999 strains.Compared with the DNA sequencing method,the accuracy of gene chip method was 99.6％ for rifampin resistance and 99.8％ for isoniazid resistance.It's concluded that the gene chip technology can quickly and accurately detect rifampin and isoniazid resistance in MTB and can be used directly for the detection of sputum samples.关键词
结核分枝杆菌/利福平/异烟肼/耐药性/基因芯片Key words
Mycobacterium tuberculosis/rifampin/isoniazid/drug resistance/gene chip分类
医药卫生引用本文复制引用
许榕青,李丹,林银霞,黄明翔,陈新朝..基因芯片技术检测结核分枝杆菌利福平和异烟肼耐药性临床应用评价[J].中国人兽共患病学报,2017,33(1):43-48,6.基金项目
Supported by the Young Research Program of Department of Public Health of Fujian Province (No.2013-2-77)福建省卫生厅青年科研课题项目(No.2013-2-77) （No.2013-2-77）
