中国人兽共患病学报2017，Vol.33Issue(2)：98-103,6.DOI:10.3969/j.issn.1002-2694.2017.02.002
IFN-γ抗鹦鹉热衣原体急性感染保护作用研究
Protective effect of interferon-γ on Chlamydia psittaci acute infection
摘要
Abstract
We investigated the effects of IFN-γ on Chlamydia psittaci (Cps) infection.HeLa cells were treated with different concentrations of recombinant human IFN-γ (5 ng/mL,25 ng/mL,50 ng/mL) after infecting with C.psittaci 6BC,then the number and morphology of C.psittaci inclusion bodies were examined after 48 hours.C57BL/6J mice were intranasally infected with 2 × 106 IFUs C.psittaci 6BC,and intraperitoneally administrated with 10 μg recombinant murine interferon-γ 24 hours prior or post infection,then body weight,activity and survival rate were recorded.The histopathology of mice livers and lungs was analyzed by HE staining on day 5 or day10 post infection.And the chlamydial inclusion bodies were titrated in the lung homogenates of mice sacrificed on day 5 after infection.The inclusion body numbers of recombinant human IFN-γ treated groups (by 5ng/mL,25ng/mL,50ng/mL) were significantly less than that in the control group (23.8±5.1)× 106,(10± 3.58) × 106,(8.0±2.22) × 106,(43.3±11.05)× 106,respectively).And the morphology of inclusion bodies in IFN-γ treated HeLa cells was irregular and much smaller.We also found that IFN-γ could significantly improve the survival rate,reduce acute clinical manifestations and pathological injurery of lung and liver in C.psittaci respiratory tract infected mice model.So we summarized that IFN-γ can mediate strong immunological protection during acute C.psittaci early infection.关键词
干扰素-γ/鹦鹉热衣原体/急性感染/免疫保护Key words
interferon-γ/Chlamydia psittaci/acute infection/immune protection分类
医药卫生引用本文复制引用
唐国芳,陈丽丽,刘良专,王川,卢兰芬,吴移谋..IFN-γ抗鹦鹉热衣原体急性感染保护作用研究[J].中国人兽共患病学报,2017,33(2):98-103,6.基金项目
This study supported by National Natural Science Foundation (grant No.31270218) （grant No.31270218）
Zhongshan Municipal Health Bureau Research Foundation (Grant No.J2012002) （Grant No.J2012002）
Zhongshan science and technology plan project (Grant No.2015B1025) （Grant No.2015B1025）
国家自然科学基金(No.31270218) （No.31270218）
中山市卫生局科研基金(No.J2012002) （No.J2012002）
中山市科技计划项目(No.2015B1025)联合资助 （No.2015B1025）
