中国人兽共患病学报2017，Vol.33Issue(2)：110-114,5.DOI:10.3969/j.issn.1002-2694.2017.02.004
辽宁省朝阳市细粒棘球病的基因型分析
Genetic analysis of Echinococcus granulosus strain in Liaoning Province, China
摘要
Abstract
To analyze the genotype of Echinococcus granulosus found in Liaoning Province,hydatid cyst was taken from the patient's liver,DNA of cyst was isolated and mitochondrial cox1 gene was sequenced substantially.Sequence results was analyzed by Blast and compared with similar strains in GenBank.Results showed that molecular analysis confirmed the echinococcosis by identical sequence of our strain with E.granulosus (AF297617) G1 genotype,with 0.1％ difference.And the sequence of our strain was consistent with 25 other strains in different countries.In conclusion,genotyping of mitochondrial gene coxl is a very useful tool for diagnosis of echinococcosis.We first reported a strain of E.granulosus in Liaoning Province,which provide novel molecular epidemiological data of this zoonosis.关键词
细粒棘球蚴/cox1基因/基因型/分子流行病学Key words
Echinococcus granulosus/Cox1 gene/genotype/molecular epidemiology分类
医药卫生引用本文复制引用
王晓黎,李飞,李金慧,刘静,李亚彩,白杨,安春丽..辽宁省朝阳市细粒棘球病的基因型分析[J].中国人兽共患病学报,2017,33(2):110-114,5.基金项目
Supported by the National Natural Science Foundation of China (No.81370189) （No.81370189）
国家自然科学基金(No.81370189)和国家自然科学基金青年基金(No.81302271)联合资助 （No.81370189）
