中国人兽共患病学报2017，Vol.33Issue(2)：115-119,135,6.DOI:10.3969/j.issn.1002-2694.2017.02.005
4b型单核细胞增生李斯特菌内化素基因NTSN_0462的功能初探
Function of an internalin gene NTSN_0462 in Listeria monocytognes serotype 4b
摘要
Abstract
Internalins play a key role in the adhesion and invasion of L.monocytogenes into host cells.In this study,the function of internalin gene NTSN_0462 in serotype 4b Lm NTSN was probed into.Firstly,NTSN_0462 was knocked out by homology recombination,then the pathogenic characterization of mutant strain LmNTSNΔ0462 was investigated in vivo and in vitro.The growth curve showed that the deletion of NTSN_0462 gene didn't affect growth and metabolism of Lm in BHI medium.The invasion of the mutant strain into human intestinal epithelial cell line Caco-2 and human hepatocyte cell line HepG2 significantly reduced comparing with the WT strains (P＜0.001).Furthermore,the infection ability to BALB/c mouse was analyzed in vivo,compared with the WT strains,the colonization of LmNTSNΔ0462 in liver decreased significantly (P＜0.001),wheareas there was no significant difference in the spleen,indicating that NTSN_0462 plays an important role in the invasion and multiplication in host livers,heretofore it is an invasion-related virulent factor of L.monocytogenes.关键词
单核细胞增生李斯特菌/NTSN_0462/血清型4b/内化素Key words
Listeria monocytogenes/NTSN_0462/serotype 4/internalin分类
医药卫生引用本文复制引用
赵丹,姚浩,蔡雪薛,孔苏伟,恽茜,谈卫军,潘志明,殷月兰,焦新安..4b型单核细胞增生李斯特菌内化素基因NTSN_0462的功能初探[J].中国人兽共患病学报,2017,33(2):115-119,135,6.基金项目
Supported by the National Natural Science Foundation of China (Nos.31472193 & 31101841) and the Priority Academic Program Development of Jiangsu Higher Education Institutions （Nos.31472193 & 31101841）
国家自然科学基金(Nos.31472193,31101841),江苏高校优势学科建设工程项目资助 （Nos.31472193,31101841）
