中国人兽共患病学报2017，Vol.33Issue(2)：126-130,5.DOI:10.3969/j.issn.1002-2694.2017.02.007
半抗原-琼脂扩散试验鉴别布鲁氏菌感染抗体和S2、Rev.1免疫抗体的研究与应用
Application of native hapten-gel diffusion test in differential diagnosis of Brucella-infected antibody and S2 or Rev.1-vaccinated antibody
摘要
Abstract
The aim of the present study was to work on the efficiency of differential diagnosis of native hapten-gel diffusion assay (NH-GD) on the background of vaccination with S2 or Rev.1.The conditions of NH-GD assay was firstly optimized,its sensitivity,specificity,repeatability and ability of differential diagnosis were determined respectively,and its test result was compared with that of fluorescence polarization assay (FPA).The results showed NH-GD assay with good specificity and repeatability could differentiate Brucella-infected antibody from vaccinated antibody after vaccination with S2 or 122 days after vaccination with Rev.1.And the result of NH-GD assay was highly consistent with that of FPA,which was simple to operate and needed a few simple equipment.Therefore,NH-GD assay was a good method for sheep brucellosis surveillance in China and especially suitable for application in grass-roots areas.关键词
布鲁氏菌病/半抗原-琼脂扩散试验/鉴别诊断Key words
brucellosis/NH-GD/differential diagnosis分类
医药卫生引用本文复制引用
杨珍,尼博,田莉莉,范伟兴..半抗原-琼脂扩散试验鉴别布鲁氏菌感染抗体和S2、Rev.1免疫抗体的研究与应用[J].中国人兽共患病学报,2017,33(2):126-130,5.基金项目
Supported by the Construction Program of Modern Agricultural(cows) Industrial Technology System(CARS-37) （cows）
现代农业(奶牛)产业技术体系建设项目专项经费(CARS-37)资助 （奶牛）
