中国人兽共患病学报2017，Vol.33Issue(2)：131-135,5.DOI:10.3969/j.issn.1002-2694.2017.02.008
γ-干扰素及外源性吲哚对泌尿生殖道沙眼衣原体生长影响的探讨
Effect of interferon-γ and exogenous indole on the growth of Chlamydia trachomatis
摘要
Abstract
We investigated the effects of γ-interferon and exogenous indole on the growth of domestic dominant standard strains and clinical straìns of Chlamydia trachomatis E-UW-5/Cx,and compared with the dominant strains of D-UW-5/Cx abroad.We used DMEM-10,DMEM-10 containing 5 ng/mL recombinant human interferon gamma (referred to as DMEM-10+IFN) and DMEM-10 containing 5 ng/mL recombinant human interferon gamma and 50 μM exogenous indole (referred to as DMEM-10+IFN+IND) to culture C.trachomatis,and then we fixed it with methanol to count inclusions after 48 hours,observing the influence of r-interferon and exogenous indole on the growth of C.trachomatis standard strains(E,D) and clinical strains.Results showed that the count of Chlamydia inclusion bodies in DMEM-10+IFN group was significantly lower than others (P＜0.05);no significant difference was found (P＞0.05) between the count of DMEM-10 group between DMEM-10+IFN+IND group.There were no significant difference between the E and D standard or clinical strains (P＞0.05).Under the effect of IFN-γ,the growth of domestic dominant strain E-UW-5/Cx C.trachomatis was significantly inhibited.After adding exogenous indole,C.trachomatis can escape the scavenging activity of IFN-γγto restore the infection vitality.关键词
沙眼衣原体/Hela细胞/γ-干扰素/外源性吲哚Key words
Chlamydia trachomatis/Hela cell/interferon-γ/exogenous indole分类
医药卫生引用本文复制引用
刘志超,刘原君,刘建刚,刘全忠..γ-干扰素及外源性吲哚对泌尿生殖道沙眼衣原体生长影响的探讨[J].中国人兽共患病学报,2017,33(2):131-135,5.基金项目
Supported by the Shandong Provincial Natural Science Foundation,China(No.ZR2011HL009)and the Tai'an Science and Technology Development Program(No.2016NS1167) （No.ZR2011HL009）
山东省自然科学基金项目(No.ZR2011HL009)和泰安市科技发展计划项目(No.2016NS1167)联合资助 （No.ZR2011HL009）
