中国人兽共患病学报2017，Vol.33Issue(2)：170-172,177,4.DOI:10.3969/j.issn.1002-2694.2017.02.014
从中哈边境亚洲璃眼蜱中检测到弗朗西斯氏菌样内共生体核酸
First detection of Francisella-like endosymbionts in Hyalomma asiaticum asiaticum from China-Kazakhstan border
摘要
Abstract
We conducted the detection the Francisella spp.nucle acid from Hyalomma asiaticum asiaticum that main distribution is on railway line area from China-Kazakhstan border.The free-living ticks were collected and then identified by morphological and molecular methods.After species identification,they were detected by PCR targeting 16S rRNA and sdhA of Francisella spp.The amplified products were sequenced and the sequences was analyzed by using the Blast.A phylogenetic tree was constructed using MEGA 6 software.A total of 243 fleas were identified as H.asiaticum asiaticum.Only 35 samples were detected for Francisella spp.positive and the positive rate was 14.4％.Sequence analysis showed that two different sequences (seql and seq2) and all belong to Francisella-like endosymbionts (FLEs).Phylogenetic analyses showed that two FLEs were belong to the same cladd.This is first detection of FLEs nucleic acid from H.asiaticum Railway line area of China-Kazakhstan border.关键词
亚洲璃眼蜱/PCR/弗朗西斯菌/中哈边境Key words
Hyalomma asiaticum asiaticum/PCR/Francisella spp./China-Kazakh border分类
医药卫生引用本文复制引用
尹小平,赵姗姗,刘丹,田延河,巴特尔,程天立,张江国,王远志..从中哈边境亚洲璃眼蜱中检测到弗朗西斯氏菌样内共生体核酸[J].中国人兽共患病学报,2017,33(2):170-172,177,4.基金项目
General administration of quality supervision,inspection and quarantine technology fund project (2016lk264) （2016lk264）
国家质检总局科技基金项目(No.2016lk264)资助 （No.2016lk264）
