中国兽医科学2017，Vol.47Issue(3)：269-278,10.DOI:10.16656/j.issn.1673-4696.2017.03.001
山羊痘病毒P32抗原基因口服活载体DNA疫苗的构建
Construction of an oral live vector DNA vaccine encoding antigen P32 gene of goatpox virus
摘要
Abstract
A novel oral live vector DNA vaccine encoding a P32 of goatpox virus without the transmembrane and hydrophobic domains,was constructed by using recombinant attenuated Salmonella typhimuriumX4550.Its safety,stability,immunogenicity and immune effects were detected and evaluated comprehensively by continuous cultivation in vitro,megadose inoculation test,immunohistochemical test,ELISA,etc.The results showed that X4550/pVAX1-P32 was of safety and stability in vitro and in vivo for BALB/c mice,and the survival rate reached to 100％ after oral immunization with 1 × 1010 CFU X4550/pVAX1-P32.The X4550/pVAX1-P32 vaccine could be passaged stably above 100 generations and recombinant S.typhimurium was isolated from the spleen 10 days post-immunization.The attenuated S.typhimurium X4550 could delivery DNA to immunologic effector cells effectively and express bioactive P32 protein.Tawny particles were detected in the spleen cells from the immunized mice by immunohistochemistry test.The results of ELISA showed that X4550/pVAX1-P32 induced a longer persistent period(P＜0.01) and higher antibody level than pVAX1-P32(0.01＜P＜0.05).These results indicate that the live vector DNA vaccine has good immunogenicity and can induce a lasting and high-level immune response,providing a basis for development of novel vaccines against goatpox.关键词
山羊痘病毒/P32基因/X4550/pVAX1-P32/免疫效果Key words
goatpox virus/P32 gene/X4550/pVAX1-P32/immune effect分类
农业科技引用本文复制引用
丁军涛,王颖,张雪彬,吴金恩,郑亚东,孔贺磊,张贝贝..山羊痘病毒P32抗原基因口服活载体DNA疫苗的构建[J].中国兽医科学,2017,47(3):269-278,10.基金项目
新疆维吾尔自治区科技支疆项目(210-60879) （210-60879）
新疆维吾尔自治区高层次引进人才项目(1101/45051) （1101/45051）
