中国兽医科学2017，Vol.47Issue(3)：279-285,7.DOI:10.16656/j.issn.1673-4696.2017.03.002
肠炎沙门菌sopE2基因缺失株的构建及其生物学活性的鉴定
Construction and characterization of a sopE2 mutant of Salmonella enteritidis C50336 strain
摘要
Abstract
To investigate the role of a sopE 2 gene from Salmonella enteritidis in biological characteristics and reveal the functional difference between sopE2 and sopE effector,we deleted the sopE2 gene in Salmonella enteritidis strain C50336 using λ-Red recombinant system,and obtained a complementary strain by transforming plasmid pBR322-sopE2 into the mutant strain.The biological characteristics including growth property,biochemical property,ability of adhesion and invasion to human epithelial cells Caco-2 BBE andmouse macrophageRAW264.7,andmedian lethal dose (LD50) formouse were then compared among the three strains.0ur results showed that there was no obvious difference in growth property and biochemical properties among those three strains.The in-vitro infection analysis showed that no significant difference was detected in the adhesion and invasion ability among those stains.For Caco-2 BBE cells,the adhesion and invasion rate was approximately 20％ and 10％,respectively.But for RAW264.7 cells,the adhesionand invasion rate reached approximately 55％o and 20％,respectively.Furthermore,the study of LD50 revealed that deletion of sopE2 did not cause the decreased virulence to mouse.The above-mentioned results provides the foundation for studying the role of sopE2 in the process of Salmonella enteritidis infection.关键词
肠炎沙门菌/sopE2基因缺失/生物学活性鉴定Key words
Salmonella enteritidis/sopE2 gene deletion/biochemical identification分类
农业科技引用本文复制引用
蔺志杰,汤佩佩,陶明新,陈韵,耿士忠,潘志明,李求春,焦新安..肠炎沙门菌sopE2基因缺失株的构建及其生物学活性的鉴定[J].中国兽医科学,2017,47(3):279-285,7.基金项目
国家自然科学基金项目(31320103907) （31320103907）
江苏省高校研究生科研创新计划项目(KYZZ_0368) （KYZZ_0368）
